Transcript: Human NR_024330.1

Homo sapiens sperm acrosome associated 6 (SPACA6), transcript variant 2, non-coding RNA.

Source:
NCBI, updated 2018-06-03
Taxon:
Homo sapiens (human)
Gene:
SPACA6 (147650)
Length:
2017
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_024330.1
NBCI Gene record:
SPACA6 (147650)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_024330.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000138747 CGCATCTGCCAGATGTTTGTT pLKO.1 302 3UTR 100% 5.625 3.938 N SPACA6 n/a
2 TRCN0000133987 CATGAGAACAACAGAGACTTA pLKO.1 527 3UTR 100% 4.950 3.465 N SPACA6 n/a
3 TRCN0000138551 CCAAAGGAGGAGATCACCTAT pLKO.1 1453 3UTR 100% 4.950 3.465 N SPACA6 n/a
4 TRCN0000137605 CAAGCTTGAAGAGTGTGAGGA pLKO.1 337 3UTR 100% 2.640 1.848 N SPACA6 n/a
5 TRCN0000138648 CGAAATCAGTGAGGAGACCAT pLKO.1 394 3UTR 100% 2.640 1.848 N SPACA6 n/a
6 TRCN0000138453 CCATCCACACTTCATCAGTGT pLKO.1 411 3UTR 100% 0.264 0.185 N SPACA6 n/a
7 TRCN0000138294 CAGAGGAGAAAGGCTCAAAGA pLKO.1 505 3UTR 100% 4.950 2.970 N SPACA6 n/a
8 TRCN0000138882 GAGGGAATAGAGACCCAGAAA pLKO.1 814 3UTR 100% 4.950 2.970 N SPACA6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_024330.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10552 pDONR223 100% 31.3% None 1_187del;235C>N;821_2017del n/a
2 ccsbBroad304_10552 pLX_304 0% 31.3% V5 1_187del;235C>N;821_2017del n/a
3 TRCN0000479620 GTGGATGCGCTCGTGATGAAAAAC pLX_317 60.2% 31.3% V5 1_187del;235C>N;821_2017del n/a
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