Construct: ORF TRCN0000479656
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014677.2_s317c1
- Derived from:
- ccsbBroadEn_10859
- DNA Barcode:
- AGTCCCTATTAAAGCTTCGGTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPX4 (2879)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479656
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2879 | GPX4 | glutathione peroxidase 4 | NM_001367832.1 | 40.5% | 16.4% | 1_303del |
| 2 | human | 2879 | GPX4 | glutathione peroxidase 4 | NM_002085.5 | 35% | 12.5% | 1_384del |
| 3 | human | 2879 | GPX4 | glutathione peroxidase 4 | NM_001039847.3 | 30.3% | 12.5% | 1_384del;500_521del;614_681del |
| 4 | human | 2879 | GPX4 | glutathione peroxidase 4 | NM_001039848.4 | 29.4% | 9% | 1_495del |
| 5 | mouse | 625249 | Gpx4 | glutathione peroxidase 4 | NM_008162.3 | 30.9% | 12.3% | (many diffs) |
| 6 | mouse | 625249 | Gpx4 | glutathione peroxidase 4 | NM_001037741.3 | 24.1% | 8.4% | (many diffs) |
| 7 | mouse | 625249 | Gpx4 | glutathione peroxidase 4 | NR_110342.1 | 20.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 212
- ORF end:
- 419
- ORF length:
- 207
- Sequence:
-
1 TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC 61 TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG 121 CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA 181 TTGATCAACA AGTTTGTACA AAAAAGTTGG CATGTTCAGC AAGATCTGCG TGAACGGGGA 241 CGACGCCCAC CCGCTGTGGA AGTGGATGAA GATCCAACCC AAGGGCAAGG GCATCCTGGG 301 AAATGCCATC AAGTGGAACT TCACCAAGTT CCTCATCGAC AAGAACGGCT GCGTGGTGAA 361 GCGCTACGGA CCCATGGAGG AGCCCCTGGT GATAGAGAAG GACCTGCCCC ACTATTTCTA 421 CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG 481 TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT 541 TTATATATCT TGTGGAAAGG ACGAAGTCCC TATTAAAGCT TCGGTACGAC GCGTTAAGTC 601 gacaatcaac ctctggatta caaaatttgt gaaagatt