Construct: ORF TRCN0000479663
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002993.1_s317c1
- Derived from:
- ccsbBroadEn_09810
- DNA Barcode:
- GAAATAGAATTATTACTGATTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL22L1 (200916)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479663
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 200916 | RPL22L1 | ribosomal protein L22 like 1 | NM_001099645.2 | 99.7% | 100% | 132C>T |
| 2 | human | 200916 | RPL22L1 | ribosomal protein L22 like 1 | NM_001320451.2 | 98.9% | 99.1% | 8_9insGCA;129C>T |
| 3 | human | 200916 | RPL22L1 | ribosomal protein L22 like 1 | NR_135259.2 | 18.5% | (many diffs) | |
| 4 | mouse | 68028 | Rpl22l1 | ribosomal protein L22 like 1 | NM_026517.2 | 92.8% | 95.9% | (many diffs) |
| 5 | mouse | 68028 | Rpl22l1 | ribosomal protein L22 like 1 | NM_001347226.1 | 92% | 95% | (many diffs) |
| 6 | mouse | 100042049 | Gm15421 | predicted gene 15421; ribos... | NR_004442.1 | 41.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 435
- ORF length:
- 366
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgccgcag aaagacagga agcccaagag gtcaacctgg aggtttaatt 121 tggaCCTTAC TCATCCAGTA GAAGATGGAA TTTTTGATTC TGGAAATTTT GAGCAATTTC 181 TACGGGAGAA GGTTAAAGTT AATGGCAAAA CTGGAAATCT CGGGAATGTT GTTCACATTG 241 AACGCTTCAA GAATAAAATC ACAGTTGTTT CTGAGAAACA GTTCTCTAAA AGGTATTTGA 301 AATACCTTAC CAAGAAATAC CTTAAGAAGA ACAATCTTCG TGATTGGCTT CGAGTGGTTG 361 CATCTGACAA GGAGACCTAC GAACTTCGTT ACTTCCAGAT TAGTCAAGAT GAAGATGAAT 421 CAGAGTCGGA GGACTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 481 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 541 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GAAATAGAAT TATTACTGAT 601 TTGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt