Construct: ORF TRCN0000479664
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001610.1_s317c1
- Derived from:
- ccsbBroadEn_08438
- DNA Barcode:
- TGTGAATACTGTGATATTGAGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A38 (54977)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479664
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54977 | SLC25A38 | solute carrier family 25 me... | NM_017875.4 | 99.8% | 100% | 165G>A |
| 2 | human | 54977 | SLC25A38 | solute carrier family 25 me... | XM_011533869.2 | 94.2% | 92.4% | (many diffs) |
| 3 | human | 54977 | SLC25A38 | solute carrier family 25 me... | XM_024453611.1 | 93.4% | 92.4% | (many diffs) |
| 4 | human | 54977 | SLC25A38 | solute carrier family 25 me... | XM_006713214.2 | 92.6% | 89.8% | 0_1ins40;29_56del;153G>A |
| 5 | human | 54977 | SLC25A38 | solute carrier family 25 me... | NM_001354798.2 | 77.8% | 68.3% | 165G>A;625_626ins167;711_712ins34 |
| 6 | mouse | 208638 | Slc25a38 | solute carrier family 25, m... | XM_006512019.3 | 80% | 77.1% | (many diffs) |
| 7 | mouse | 208638 | Slc25a38 | solute carrier family 25, m... | NM_144793.1 | 77.9% | 80.9% | (many diffs) |
| 8 | mouse | 208638 | Slc25a38 | solute carrier family 25, m... | XM_006512020.1 | 58.5% | 60.1% | (many diffs) |
| 9 | mouse | 208638 | Slc25a38 | solute carrier family 25, m... | XM_011242949.1 | 58.5% | 60.1% | (many diffs) |
| 10 | mouse | 208638 | Slc25a38 | solute carrier family 25, m... | XM_011242950.2 | 58.5% | 60.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 978
- ORF length:
- 912
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tcagaactca cgtccgtcgc tgctgcaacc ccaagatgtc ggagacacgg 121 tggaaacgct tatgttacat ccggtgatca aggctttcct gtgtggctcc atcagtggga 181 cctgctctac cctccttttc caacctctgg atctccttaa aacacgccta caaaccctcc 241 agccctcaga tcatgggtct agacgtgttg ggatgttggc tgtactcttg aaggtggttc 301 gcacggagag tcttttgggc ctttggaaag ggatgtcccc ttccattgtg agatgtgtcc 361 ctggcgttgg aatctacttt ggcactctct actctttgaa gcagtatttc ttgcgaggcc 421 atcccccaac cgccctggag tcagtcatgc tgggggtggg ctctcgctct gttgcagggg 481 tctgtatgtc acctatcact gtaatcaaga cgcgctatga gagtgggaaa tatggctatg 541 agagtatcta cgctgccctg aggagcatct atcacagtga ggggcaccgg ggcctcttca 601 gtggcctgac agcaactctc cTTCGAGATG CGCCCTTCTC AGGAATCTAC CTGATGTTTT 661 ACAACCAGAC CAAAAATATA GTGCCTCATG ACCAGGTGGA TGCAACCCTT ATTCCTATTA 721 CAAATTTCAG CTGTGGGATA TTTGCTGGTA TTCTGGCCTC ACTGGTAACT CAACCTGCGG 781 ATGTTATCAA AACTCATATG CAGCTTTATC CACTGAAGTT TCAATGGATT GGCCAAGCAG 841 TGACACTTAT TTTCAAAGAC TATGGACTAC GTGGCTTCTT CCAAGGTGGC ATCCCCCGAG 901 CCCTCCGCAG AACTCTAATG GCAGCAATGG CGTGGACGGT GTATGAAGAG ATGATGGCCA 961 AGATGGGCCT GAAGTCCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1021 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1081 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGTGAAT ACTGTGATAT 1141 TGAGTTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt