Construct: ORF TRCN0000479672
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017225.3_s317c1
- Derived from:
- ccsbBroadEn_14651
- DNA Barcode:
- ATTGATCGCCACCAACGTAGACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALK1 (2584)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479672
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2584 | GALK1 | galactokinase 1 | NM_000154.2 | 99.9% | 100% | 189G>A |
| 2 | mouse | 14635 | Galk1 | galactokinase 1 | NM_016905.2 | 85.4% | 88% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1242
- ORF length:
- 1176
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgctttgaga cagccccagg tcgcggagct gctggccgag gcccggcgag 121 ccttccggga ggagttcggg gccgagcccg agctggccgt gtcagcgccg ggccgcgtca 181 acctcatcgg ggaacacacg gactacaacc agggcctggt gctgcctatg gctctggagc 241 tcatgacggt gctagtgggc agcccccgca aggatgggct ggtgtctctc ctcaccacct 301 ctgagggtgc cgatgagccc cagcggctgc agtttccact gcccacagcc cagcgctcgc 361 tggagcctgg gactcctcgg tgggccaact atgtcaaggg agtgattcag tactacccag 421 ctgcccccct ccctggcttc agtgcagtgg tggtcagctc agtgcccctg gggggtggcc 481 tgtccagctc agcatccttg gaagtggcca cgtacacctt cctccagcag ctctgtccag 541 actcgggcac aatagctgcc cgcgcccagg tgtgtcagca ggccgagcac agcttcgcag 601 ggatgccctg tggcatcatg gaccagttca tctcacttat gggacagaaa ggccacgcgc 661 tgctcattga ctgcaggtcc ttggagacca gcctggtgcc actctcggac cccaagctgg 721 ccgtgctcat caccaactct aatgtccgcc actccctggc ctccagcgag taccctgtgc 781 ggcggcgcca atgtgaagaa gtggcccggg cgctgggcaa ggaaagcctc cgggaggtac 841 aactggaaga gctagaggct gccagggacc tggtgagcaa agagggcttc cggcgggccc 901 ggcacgtggt gggggagatt cggcgcacgg cccaggcagc ggccgcccTG AGACGTGGCG 961 ACTACAGAGC CTTTGGCCGC CTCATGGTGG AGAGCCACCG CTCACTCAGA GACGACTATG 1021 AGGTGAGCTG CCCAGAGCTG GACCAGCTGG TGGAGGCTGC GCTTGCTGTG CCTGGGGTTT 1081 ATGGCAGCCG CATGACGGGC GGTGGCTTCG GTGGCTGCAC GGTGACACTG CTGGAGGCCT 1141 CCGCTGCTCC CCACGCCATG CGGCACATCC AGGAGCACTA CGGCGGGACT GCCACCTTCT 1201 ACCTCTCTCA AGCAGCCGAT GGAGCCAAGG TGCTGTGCTT GTGCCCAACT TTCTTGTACA 1261 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1321 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1381 AGGACGAATT GATCGCCACC AACGTAGACC TACGCGTTAA GTCgacaatc aacctctgga 1441 ttacaaaatt tgtgaaagat t