Construct: ORF TRCN0000479677
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012215.1_s317c1
- Derived from:
- ccsbBroadEn_00167
- DNA Barcode:
- AAAACCCGAACGCCTTCTGATTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BMP7 (655)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479677
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 655 | BMP7 | bone morphogenetic protein 7 | NM_001719.3 | 100% | 100% | |
| 2 | mouse | 12162 | Bmp7 | bone morphogenetic protein 7 | NM_007557.3 | 90.7% | 97.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1359
- ORF length:
- 1293
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca cgtgcgctca ctgcgagctg cggcgccgca cagcttcgtg gcgctctggg 121 cacccctgtt cctgctgcgc tccgccctgg ccgacttcag cctggacaac gaggtgcact 181 cgagcttcat ccaccggcgc ctccgcagcc aggagcggcg ggagatgcag cgcgagatcc 241 tctccatttt gggcttgccc caccgcccgc gcccgcacct ccagggcaag cacaactcgg 301 cacccatgtt catgctggac ctgtacaacg ccatggcggt ggaggagggc ggcgggcccg 361 gcggccaggg cttctcctac ccctacaagg ccgtcttcag tacccagggc ccccctctgg 421 ccagcctgca agatagccat ttcctcaccg acgccgacat ggtcatgagc ttcgtcaacc 481 tcgtggaaca tgacaaggaa ttcttccacc cacgctacca ccatcgagag ttccggtttg 541 atctttccaa gatcccagaa ggggaagctg tcacggcagc cgaattccgg atctacaagg 601 actacatccg ggaacgcttc gacaatgaga cgttccggat cagcgtttat caggtgctcc 661 aggagcactt gggcagggaa tcggatctct tcctgctcga cagccgtacc ctctgggcct 721 cggaggaggg ctggctggtg tttgacatca cagccaccag caaccactgg gtggtcaatc 781 cgcggcacaa cctgggcctg cagctctcgg tggagacgct ggatgggcag agcatcaacc 841 ccaagttggc gggcctgatt gggcggcacg ggccccagaa caagcagccc ttcatggtgg 901 ctttcttcaa ggccacggag gtccacttcc gcagcatccg gtccacgggg agcaaacagc 961 gcagccagaa ccgctccaag acgcccaaga accaggaagc ccTGCGGATG GCCAACGTGG 1021 CAGAGAACAG CAGCAGCGAC CAGAGGCAGG CCTGTAAGAA GCACGAGCTG TATGTCAGCT 1081 TCCGAGACCT GGGCTGGCAG GACTGGATCA TCGCGCCTGA AGGCTACGCC GCCTACTACT 1141 GTGAGGGGGA GTGTGCCTTC CCTCTGAACT CCTACATGAA CGCCACCAAC CACGCCATCG 1201 TGCAGACGCT GGTCCACTTC ATCAACCCGG AAACGGTGCC CAAGCCCTGC TGTGCGCCCA 1261 CGCAGCTCAA TGCCATCTCC GTCCTCTACT TCGATGACAG CTCCAACGTC ATCCTGAAGA 1321 AATACAGAAA CATGGTGGTC CGGGCCTGTG GCTGCCACTA CCCAACTTTC TTGTACAAAG 1381 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1441 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1501 ACGAAAAACC CGAACGCCTT CTGATTGCAC GCGTTAAGTC gacaatcaac ctctggatta 1561 caaaatttgt gaaagatt