Construct: ORF TRCN0000479687
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012829.1_s317c1
- Derived from:
- ccsbBroadEn_01738
- DNA Barcode:
- GTCCTCAAGCTGACAACTGCAAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE2G1 (7326)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479687
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7326 | UBE2G1 | ubiquitin conjugating enzym... | NM_003342.4 | 100% | 100% | |
| 2 | mouse | 67128 | Ube2g1 | ubiquitin-conjugating enzym... | NM_025985.4 | 95.2% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 576
- ORF length:
- 510
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ggagctgcag tcggcactgc tactgcgaag acagctggca gaactcaaca 121 aaaatccagt ggaaggcttt tcTGCAGGTT TAATAGATGA CAATGATCTC TACCGATGGG 181 AAGTCCTTAT TATTGGCCCT CCAGATACAC TTTATGAAGG TGGTGTTTTT AAGGCTCATC 241 TTACTTTCCC AAAAGATTAT CCCCTCCGAC CTCCTAAAAT GAAATTCATT ACAGAAATCT 301 GGCACCCAAA TGTTGATAAA AATGGTGATG TGTGCATTTC TATTCTTCAT GAGCCTGGGG 361 AAGATAAGTA TGGTTATGAA AAGCCAGAGG AACGCTGGCT CCCTATCCAC ACTGTGGAAA 421 CCATCATGAT TAGTGTCATT TCTATGCTGG CAGACCCTAA TGGAGACTCA CCTGCTAATG 481 TTGATGCTGC GAAAGAATGG AGGGAAGATA GAAATGGAGA ATTTAAAAGA AAAGTTGCCC 541 GCTGTGTAAG AAAAAGCCAA GAGACTGCTT TTGAGTGCCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 AGTCCTCAAG CTGACAACTG CAAGAACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt