Construct: ORF TRCN0000479714
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004483.4_s317c1
- Derived from:
- ccsbBroadEn_14802
- DNA Barcode:
- CTTCCCTCTGGAGACCCTCGTTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAPK6 (5597)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479714
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5597 | MAPK6 | mitogen-activated protein k... | NM_002748.4 | 99.9% | 99.8% | 868C>G |
| 2 | human | 5597 | MAPK6 | mitogen-activated protein k... | XM_005254537.3 | 99.9% | 99.8% | 868C>G |
| 3 | human | 5597 | MAPK6 | mitogen-activated protein k... | XM_005254538.2 | 99.9% | 99.8% | 868C>G |
| 4 | human | 5597 | MAPK6 | mitogen-activated protein k... | XM_011521782.1 | 99.9% | 99.8% | 868C>G |
| 5 | mouse | 50772 | Mapk6 | mitogen-activated protein k... | NM_015806.5 | 87.7% | 94.4% | (many diffs) |
| 6 | mouse | 50772 | Mapk6 | mitogen-activated protein k... | NM_027418.2 | 87.7% | 94.4% | (many diffs) |
| 7 | mouse | 50772 | Mapk6 | mitogen-activated protein k... | XM_011242767.2 | 87.7% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2229
- ORF length:
- 2163
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agagaaattt gaaagtctca tgaacattca tggttttgat ctgggttcta 121 ggtatatgga cttaaaacca ttgggttgtg gaggcaatgg cttggttttt tctgctgtag 181 acaatgactg tgacaaaaga gtagccatca agaaaattgt ccttactgat ccccagagtg 241 tcaaacatgc tctacgtgaa atcaaaatta ttagaagact tgaccatgat aacattgtga 301 aagtgtttga gattcttggt cccagtggaa gccaattaac agacgatgtg ggctctctta 361 cggaactgaa cagtgtttac attgttcagg agtacatgga gacagacttg gctaatgtgc 421 tggagcaggg ccctttactg gaagagcatg ccaggctttt catgtatcag ctgctacggg 481 ggctcaagta tattcactct gcaaatgtac tgcacagaga tctcaaacca gctaatcttt 541 tcattaatac ggaagacttg gtgctgaaga taggtgactt tggtcttgca cggatcatgg 601 atcctcatta ttcccataag ggtcatcttt ctgaaggatt ggttactaaa tggtacagat 661 ctccacgtct tttactttct cctaataatt atactaaagc cattgacatg tgggctgcag 721 gctgcatctt tgctgaaatg ctgactggta aaaccctttt tgcaggtgca catgaacttg 781 aacagatgca gctgatttta gaatctattc ctgttgtaca tgaggaagat cgtcaggagc 841 ttctcagcgt aattccagtt tacattagaa atgacatgac tgagccacac aaacctttaa 901 ctcagctgct tccaggaatt agtcgagaag cagtggattt cctggaacaa attttgacat 961 ttagccccat ggatcggtta acagcagaag aagcactctc ccatccttac atgagcatat 1021 attcttttcc aatggatgag ccaatttcaa gccatccttt tcatattgaa gatgaagttg 1081 atgatatttt gcttatggat gaaactcaca gtcacattta taactgggaa aggtatcatg 1141 attgtcagtt ttcagagcat gattggcctg tacataacaa ctttgatatt gatgaagttc 1201 agcttgatcc aagagctctg tccgatgtca ctgatgaaga agaagtacaa gttgatcccc 1261 gaaaatattt ggatggagat cgggaaaagt atctggagga tcctgctttt gataccaatt 1321 actctactga gccttgttgg caatactcag atcatcatga aaacaaatat tgtgatctgg 1381 agtgtagcca tacttgtaac tacaaaacga ggtcatcatc atatttagat aacttagttt 1441 ggagagagag tgaagttaac cattactatg aacccaagct tattatagat ctttccaatt 1501 ggaaagaaca aagcaaagaa aaatctgata agaaaggcaa atcaaaatgt gaaaggaatg 1561 gattggttaa agcccagata gcgctagagg aagcatcaca gcaactggct ggaaaagaaa 1621 gggaaaagaa tcagggattt gattttgatt cctttattgc aggaactatt cagcttagtt 1681 cccagcatga gcctactgat gttgttgata aattaaatga cttgaatagc tcagtgtccc 1741 aactagaatt gaaaagtttg atatcaaagt cagtaagcca agaaaaacag gaaaaaggaa 1801 tggcaaatct ggctcaatta gaagccttgt accagtcttc ttgggacagc cagtttgtga 1861 gtggtgggga ggactgtttt TTCATAAATC AGTTTTGTGA GGTAAGGAAG GATGAACAAG 1921 TTGAGAAGGA AAACACTTAC ACTAGTTACT TGGACAAGTT CTTTAGCAGG AAAGAAGATA 1981 CTGAAATGCT AGAAACTGAG CCAGTAGAGG ATGGGAAGCT TGGGGAGAGA GGACATGAGG 2041 AAGGATTTCT GAACAACAGT GGGGAGTTCC TCTTTAACAA GCAGCTCGAG TCCATAGGCA 2101 TCCCACAGTT TCACAGTCCA GTTGGGTCAC CACTTAAGTC AATACAGGCC ACATTAACAC 2161 CTTCTGCTAT GAAATCTTCC CCTCAAATTC CTCATCAAAC ATACAGCAGC ATTCTGAAAC 2221 ATCTGAACTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2281 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2341 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTTCCC TCTGGAGACC CTCGTTCTAC 2401 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt