Construct: ORF TRCN0000479714

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004483.4_s317c1
Derived from:
ccsbBroadEn_14802
DNA Barcode:
CTTCCCTCTGGAGACCCTCGTTCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAPK6 (5597)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479714
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5597 MAPK6 mitogen-activated protein k... NM_002748.4 99.9% 99.8% 868C>G
2 human 5597 MAPK6 mitogen-activated protein k... XM_005254537.3 99.9% 99.8% 868C>G
3 human 5597 MAPK6 mitogen-activated protein k... XM_005254538.2 99.9% 99.8% 868C>G
4 human 5597 MAPK6 mitogen-activated protein k... XM_011521782.1 99.9% 99.8% 868C>G
5 mouse 50772 Mapk6 mitogen-activated protein k... NM_015806.5 87.7% 94.4% (many diffs)
6 mouse 50772 Mapk6 mitogen-activated protein k... NM_027418.2 87.7% 94.4% (many diffs)
7 mouse 50772 Mapk6 mitogen-activated protein k... XM_011242767.2 87.7% 94.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2229
ORF length:
2163
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc agagaaattt gaaagtctca tgaacattca tggttttgat ctgggttcta
121ggtatatgga cttaaaacca ttgggttgtg gaggcaatgg cttggttttt tctgctgtag
181acaatgactg tgacaaaaga gtagccatca agaaaattgt ccttactgat ccccagagtg
241tcaaacatgc tctacgtgaa atcaaaatta ttagaagact tgaccatgat aacattgtga
301aagtgtttga gattcttggt cccagtggaa gccaattaac agacgatgtg ggctctctta
361cggaactgaa cagtgtttac attgttcagg agtacatgga gacagacttg gctaatgtgc
421tggagcaggg ccctttactg gaagagcatg ccaggctttt catgtatcag ctgctacggg
481ggctcaagta tattcactct gcaaatgtac tgcacagaga tctcaaacca gctaatcttt
541tcattaatac ggaagacttg gtgctgaaga taggtgactt tggtcttgca cggatcatgg
601atcctcatta ttcccataag ggtcatcttt ctgaaggatt ggttactaaa tggtacagat
661ctccacgtct tttactttct cctaataatt atactaaagc cattgacatg tgggctgcag
721gctgcatctt tgctgaaatg ctgactggta aaaccctttt tgcaggtgca catgaacttg
781aacagatgca gctgatttta gaatctattc ctgttgtaca tgaggaagat cgtcaggagc
841ttctcagcgt aattccagtt tacattagaa atgacatgac tgagccacac aaacctttaa
901ctcagctgct tccaggaatt agtcgagaag cagtggattt cctggaacaa attttgacat
961ttagccccat ggatcggtta acagcagaag aagcactctc ccatccttac atgagcatat
1021attcttttcc aatggatgag ccaatttcaa gccatccttt tcatattgaa gatgaagttg
1081atgatatttt gcttatggat gaaactcaca gtcacattta taactgggaa aggtatcatg
1141attgtcagtt ttcagagcat gattggcctg tacataacaa ctttgatatt gatgaagttc
1201agcttgatcc aagagctctg tccgatgtca ctgatgaaga agaagtacaa gttgatcccc
1261gaaaatattt ggatggagat cgggaaaagt atctggagga tcctgctttt gataccaatt
1321actctactga gccttgttgg caatactcag atcatcatga aaacaaatat tgtgatctgg
1381agtgtagcca tacttgtaac tacaaaacga ggtcatcatc atatttagat aacttagttt
1441ggagagagag tgaagttaac cattactatg aacccaagct tattatagat ctttccaatt
1501ggaaagaaca aagcaaagaa aaatctgata agaaaggcaa atcaaaatgt gaaaggaatg
1561gattggttaa agcccagata gcgctagagg aagcatcaca gcaactggct ggaaaagaaa
1621gggaaaagaa tcagggattt gattttgatt cctttattgc aggaactatt cagcttagtt
1681cccagcatga gcctactgat gttgttgata aattaaatga cttgaatagc tcagtgtccc
1741aactagaatt gaaaagtttg atatcaaagt cagtaagcca agaaaaacag gaaaaaggaa
1801tggcaaatct ggctcaatta gaagccttgt accagtcttc ttgggacagc cagtttgtga
1861gtggtgggga ggactgtttt TTCATAAATC AGTTTTGTGA GGTAAGGAAG GATGAACAAG
1921TTGAGAAGGA AAACACTTAC ACTAGTTACT TGGACAAGTT CTTTAGCAGG AAAGAAGATA
1981CTGAAATGCT AGAAACTGAG CCAGTAGAGG ATGGGAAGCT TGGGGAGAGA GGACATGAGG
2041AAGGATTTCT GAACAACAGT GGGGAGTTCC TCTTTAACAA GCAGCTCGAG TCCATAGGCA
2101TCCCACAGTT TCACAGTCCA GTTGGGTCAC CACTTAAGTC AATACAGGCC ACATTAACAC
2161CTTCTGCTAT GAAATCTTCC CCTCAAATTC CTCATCAAAC ATACAGCAGC ATTCTGAAAC
2221ATCTGAACTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
2281CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
2341TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTTCCC TCTGGAGACC CTCGTTCTAC
2401GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)