Construct: ORF TRCN0000479749
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002020.1_s317c1
- Derived from:
- ccsbBroadEn_11206
- DNA Barcode:
- GCCCTCTAAAAATCTACTTCAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPYL1 (7259)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479749
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7259 | TSPYL1 | TSPY like 1 | NM_003309.4 | 99.7% | 99.7% | 521_522insGGT |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1383
- ORF length:
- 1314
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcggcctg gatggggtca agaggaccac tcccctccaa acccacagca 121 tcattatttc tgaccaagtc ccgagcgacc aggacgcaca ccagtacctg aggctccgcg 181 accaaagcga ggcgacacag gtgatggcgg agccgggtga gggaggctcg gagaccgtcg 241 cgctcccgcc tccaccgcct tcagaggagg ggggcgtacc ccaggatgcc gcgggccgtg 301 gcggtactcc ccagatccga gttgttgggg gtcgcggtca tgtggcgatc aaagccgggc 361 aggaagaggg ccagcctccc gccgaaggcc tggcagccgc ttctgtggtg atggcagccg 421 accgcagcct gaaaaagggc gttcagggtg gagagaaggc cctagaaatc tgtggcgccc 481 agagatccgc gtctgagctg acggcggggg cggaggctga ggcggaggag gtgaagacag 541 gaaagtgcgc caccgtctca gcagccgtgg ctgagaggga gagcgctgag gtggtggtga 601 aggaaggcct ggcggagaag gaggtaatgg aggagcagat ggaggtagag gagcagccgc 661 cagaaggtga agaaatagaa gtggcggagg aggatagatt ggaggaggag gcgagggagg 721 aagaagggcc ctggcctttg catgaggctc tccgcatgga ccctctggag gccatccagc 781 tggaactgga cactgtgaat gctcaggccg acagggcctt ccaacagctg gagcacaagt 841 ttgggcggat gcgtcgacac tacctggagc ggaggaacta catcattcag aatatcccgg 901 gcttctggat gactgctttt cgaaaccacc cccagttgtc cgccatgatt aggggccaag 961 atgcagagat gttaaggtac ataaccaatt tagaggtgaa ggaactcaga caccctagaa 1021 ccggttgcaa gttcaagttc ttctttagaa gaaaccccTA CTTCAGAAAC AAGCTGATTG 1081 TCAAGGAATA TGAGGTAAGA TCCTCCGGCC GAGTGGTGTC TCTTTCTACT CCAATTATAT 1141 GGCGCAGGGG GCATGAACCC CAGTCCTTCA TTCGCAGAAA CCAAGACCTC ATCTGCAGCT 1201 TCTTCACTTG GTTTTCAGAC CACAGCCTTC CAGAGTCCGA CAAAATTGCT GAGATTATTA 1261 AAGAGGATCT GTGGCCAAAT CCACTGCAAT ACTACCTGTT GCGTGAAGGA GTCCGTAGAG 1321 CCCGACGTCG CCCGCTAAGG GAGCCTGTAG AGATCCCCAG GCCCTTTGGG TTCCAGTCTG 1381 GTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1441 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1501 GGCTTTATAT ATCTTGTGGA AAGGACGAGC CCTCTAAAAA TCTACTTCAG CCACGCGTTA 1561 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt