Construct: ORF TRCN0000479750
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001570.1_s317c1
- Derived from:
- ccsbBroadEn_06259
- DNA Barcode:
- CACCCTCCCCCAGGAGTTCTAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GATA2 (2624)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479750
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2624 | GATA2 | GATA binding protein 2 | NM_001145662.1 | 99.9% | 100% | 15C>G |
| 2 | human | 2624 | GATA2 | GATA binding protein 2 | NM_001145661.2 | 97% | 97% | 15C>G;1017_1058del |
| 3 | human | 2624 | GATA2 | GATA binding protein 2 | NM_032638.5 | 97% | 97% | 15C>G;1017_1058del |
| 4 | mouse | 14461 | Gata2 | GATA binding protein 2 | NM_008090.5 | 87.3% | 95% | (many diffs) |
| 5 | mouse | 14461 | Gata2 | GATA binding protein 2 | XM_006505539.3 | 87.3% | 95% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1464
- ORF length:
- 1398
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggtggcgccg gagcagccgc gctggatggc gcacccggcc gtgctgaatg 121 cgcagcaccc cgactcacac cacccgggcc tggcgcacaa ctacatggaa cccgcgcagc 181 tgctgcctcc agacgaggtg gacgtcttct tcaatcacct cgactcgcag ggcaacccct 241 actatgccaa ccccgctcac gcgcgggcgc gcgtctccta cagccccgcg cacgcccgcc 301 tgaccggagg ccagatgtgc cgcccacact tgttgcacag cccgggtttg ccctggctgg 361 acgggggcaa agcagccctc tctgccgctg cggcccacca ccacaacccc tggaccgtga 421 gccccttctc caagacgcca ctgcacccct cagctgctgg aggccctgga ggcccactct 481 ctgtgtaccc aggggctggg ggtgggagcg ggggaggcag cgggagctca gtggcctccc 541 tcacccctac agcagcccac tctggctccc accttttcgg cttcccaccc acgccaccca 601 aagaagtgtc tcctgaccct agcaccacgg gggctgcgtc tccagcctca tcttccgcgg 661 ggggtagtgc agcccgagga gaggacaagg acggcgtcaa gtaccaggtg tcactgacgg 721 agagcatgaa gatggaaagt ggcagtcccc tgcgcccagg cctagctact atgggcaccc 781 agcctgctac acaccacccc atccccacct acccctccta tgtgccggcg gctgcccacg 841 actacagcag cggactcttc caccccggag gcttcctggg gggaccggcc tccagcttca 901 cccctaagca gcgcagcaag gctcgttcct gttcagaagg ccgggagtgt gtcaactgtg 961 gggccacagc cacccctctc tggcggcggg acggcaccgg ccactacctg tgcaatgcct 1021 gtggcctcta ccacaagatg aatgggcaga accgaccact catcaagccc aagcgaagac 1081 tgacgacaac caccacctta tggcgccgaa acgccaacgg ggaccctgtc TGCAACGCCT 1141 GTGGCCTCTA CTACAAGCTG CACAATGTTA ACAGGCCACT GACCATGAAG AAGGAAGGGA 1201 TCCAGACTCG GAACCGGAAG ATGTCCAACA AGTCCAAGAA GAGCAAGAAA GGGGCGGAGT 1261 GCTTCGAGGA GCTGTCAAAG TGCATGCAGG AGAAGTCATC CCCCTTCAGT GCAGCTGCCC 1321 TGGCTGGACA CATGGCACCT GTGGGCCACC TCCCGCCCTT CAGCCACTCC GGACACATCC 1381 TGCCCACTCC GACGCCCATC CACCCCTCCT CCAGCCTCTC CTTCGGCCAC CCCCACCCGT 1441 CCAGCATGGT GACCGCCATG GGCCACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1501 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1561 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC ACCCTCCCCC 1621 AGGAGTTCTA GATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1681 att