Construct: ORF TRCN0000479756
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018820.2_s317c1
- Derived from:
- ccsbBroadEn_15148
- DNA Barcode:
- ATTTCCTTGTTAATAGACCCAACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PANK3 (79646)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479756
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79646 | PANK3 | pantothenate kinase 3 | NM_024594.4 | 99.4% | 54.9% | (many diffs) |
| 2 | mouse | 211347 | Pank3 | pantothenate kinase 3 | NM_145962.2 | 92.9% | 55.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 756
- ORF length:
- 687
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagatcaaa gatgccaaga aaccctcttt cccatggttt ggcatggaca 121 ttgggggaac tctagtaaag ctctcgtact ttgaacctat tgatatcaca gcagaggaag 181 agcaagaaga agttgagagt ttaaaaagta ttcggaaata tttgacttct aacgtggcat 241 atggatccac cggcattcgg gatgtacacc ttgaactgaa agatttaaca ctttttggcc 301 gaagagggaa cttgcacttt atcaggtttc caacccagga cctgcctact tttatccaaa 361 tgggaagaga taaaaacttc tcaacaattg cagaacggtg ctatgtgcta caggagggtg 421 gtgcttacaa gtttgaaaaa gattttcgca caattggaaa cctccacctg cacaaactgg 481 atgaacttga ctgccttgta aagggcttgc tgtatataga ctctgtcagt ttcaatggac 541 aagccgagtg ctattatttt gctaatgcct cagaacntga gcgatgccaa aagatgcctt 601 ttaacctgga tgatccctat ccactgcttg tagtgaacat tggctcagga gtcagtattt 661 tagcagtcca ttccaaagac aactataaac gagtgactgg gacangcctt tggagggggt 721 acctttctgg gtttatgcag tttattgact ggctgtgaaa gttttGAAGA GGCTCTTGAA 781 ATGGCATCCA AAGGTGATAG CACACAAGCT GACAAGCTGG TCCGTGATAT TTATGGAGGA 841 GATTATGAAA GATTTGGTTT GCCAGGTTGG GCTGTAGCAT CTAGTTTTGG GAATATGATT 901 TATAAGGAGA AGCGAGAATC TGTTAGTAAA GAAGATCTGG CAAGAGCTAC TTTAGTTACT 961 ATCACCAATA ACATTGGTTC TGTGGCACGA ATGTGTGCTG TTAATGAGAA AATAAACAGA 1021 GTTGTCTTTG TTGGAAACTT TTTACGTGTC AATACCCTCT CAATGAAACT TTTGGCATAT 1081 GCACTGGATT ACTGGTCAAA AGGTCAACTA AAAGCATTGT TTCTAGAACA TGAGGGTTAC 1141 TTTGGAGCAG TTGGTGCACT TCTTGGGCTG CCAAATTTCA GCTTGCCAAC TTTCTTGTAC 1201 AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG 1261 TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA 1321 AAGGACGAAT TTCCTTGTTA ATAGACCCAA CAACGCGTTA AGTCgacaat caacctctgg 1381 attacaaaat ttgtgaaaga tt