Construct: ORF TRCN0000479763
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004517.1_s317c1
- Derived from:
- ccsbBroadEn_14660
- DNA Barcode:
- TAATGAACCCTGGACGGGTCTAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSK3A (2931)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479763
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2931 | GSK3A | glycogen synthase kinase 3 ... | NM_019884.3 | 99.7% | 99.3% | 15G>N;91_92insT;94delG |
| 2 | human | 2931 | GSK3A | glycogen synthase kinase 3 ... | XR_001753673.1 | 54.8% | (many diffs) | |
| 3 | mouse | 606496 | Gsk3a | glycogen synthase kinase 3 ... | NM_001031667.1 | 88.9% | 93.2% | (many diffs) |
| 4 | mouse | 606496 | Gsk3a | glycogen synthase kinase 3 ... | XM_006540258.1 | 83.6% | 86.8% | (many diffs) |
| 5 | mouse | 606496 | Gsk3a | glycogen synthase kinase 3 ... | XM_006540259.3 | 77.2% | 80.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1515
- ORF length:
- 1449
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cggcggcggn ccttcgggag gcggccctgg gggctcgggc agggcgcgga 121 ctagctcgtt cgcggagccc ggcggcggag gcggagtgag cggcggcggc cccggaggct 181 cggcctccgg cccaggcggc accggcggcg gaaaggcatc tgtcggggcc atgggtgggg 241 gcgtcggggc ctcgagctcc gggggtggac ccggcggcag cggcggagga ggcagcggag 301 gccccggcgc aggcactagc ttcccgccgc ccggggtgaa gctgggccgt gacagcggga 361 aggtgaccac agtcgtagcc actctaggcc aaggcccaga gcgctcccaa gaagtggctt 421 acacggacat caaagtgatt ggcaatggct catttggggt cgtgtaccag gcacggctgg 481 cagagaccag ggaactagtc gccatcaaga aggttctcca ggacaagagg ttcaagaacc 541 gagagctgca gatcatgcgt aagctggacc actgcaatat tgtgaggctg agatactttt 601 tctactccag tggcgagaag aaagacgagc tttacctaaa tctggtgctg gaatatgtgc 661 ccgagacagt gtaccgggtg gcccgccact tcaccaaggc caagttgacc atccctatcc 721 tctatgtcaa ggtgtacatg taccagctct tccgcagctt ggcctacatc cactcccagg 781 gcgtgtgtca ccgcgacatc aagccccaga acctgctggt ggaccctgac actgctgtcc 841 tcaagctctg cgattttggc agtgcaaagc agttggtccg aggggagccc aatgtctcct 901 acatctgttc tcgctactac cgggccccag agctcatctt tggagccact gattacacct 961 catccatcga tgtttggtca gctggctgtg tactggcaga gctcctcttg ggccagccca 1021 tcttccctgg ggacagtggg gtggaccagc tggtggagat catcaaggtg ctgggaacac 1081 caacccggga acaaatccga gagatgaacc ccaactacac ggagttcaag ttccctcaga 1141 ttaaagctca cccctggaca aaggtgttca aatCTCGAAC GCCGCCAGAG GCCATCGCGC 1201 TCTGCTCTAG CCTGCTGGAG TACACCCCAT CCTCAAGGCT CTCCCCACTA GAGGCCTGTG 1261 CGCACAGCTT CTTTGATGAA CTGCGATGTC TGGGAACCCA GCTGCCTAAC AACCGCCCAC 1321 TTCCCCCTCT CTTCAACTTC AGTGCTGGTG AACTCTCCAT CCAACCGTCT CTCAACGCCA 1381 TTCTCATCCC TCCTCACTTG AGGTCCCCAG CGGGCACTAC CACCCTCACC CCGTCCTCAC 1441 AAGCTTTAAC TGAGACTCCG ACCAGCTCAG ACTGGCAGTC GACCGATGCC ACACCTACCC 1501 TCACTAACTC CTCCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1561 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1621 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TAATGAACCC TGGACGGGTC 1681 TAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt