Construct: ORF TRCN0000479770
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013356.2_s317c1
- Derived from:
- ccsbBroadEn_14811
- DNA Barcode:
- GCAAAGCTAGGTTCCCCTATATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K7 (5609)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479770
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5609 | MAP2K7 | mitogen-activated protein k... | NM_001297556.2 | 99.7% | 99.7% | 51G>A;573C>T;1273T>C |
| 2 | human | 5609 | MAP2K7 | mitogen-activated protein k... | NM_145185.4 | 98.1% | 98.1% | (many diffs) |
| 3 | human | 5609 | MAP2K7 | mitogen-activated protein k... | XM_006722800.2 | 96.1% | 95.9% | (many diffs) |
| 4 | human | 5609 | MAP2K7 | mitogen-activated protein k... | NM_001297555.2 | 94.5% | 94.3% | (many diffs) |
| 5 | mouse | 26400 | Map2k7 | mitogen-activated protein k... | NM_011944.3 | 88.6% | 96.9% | (many diffs) |
| 6 | mouse | 26400 | Map2k7 | mitogen-activated protein k... | NM_001164172.1 | 85.4% | 93.2% | (many diffs) |
| 7 | mouse | 26400 | Map2k7 | mitogen-activated protein k... | NM_001291777.1 | 82.2% | 89.7% | (many diffs) |
| 8 | mouse | 26400 | Map2k7 | mitogen-activated protein k... | NM_001042557.2 | 79.4% | 86.5% | (many diffs) |
| 9 | mouse | 26400 | Map2k7 | mitogen-activated protein k... | NM_001291783.1 | 72.6% | 80% | (many diffs) |
| 10 | mouse | 26400 | Map2k7 | mitogen-activated protein k... | NM_001291778.1 | 67.3% | 74% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1344
- ORF length:
- 1278
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgtcctcc ctggaacaga agctgtcccg cctggaagca aagctaaagc 121 aggagaaccg ggaggcccgg cggaggatcg acctcaacct ggatatcagc ccccagcggc 181 ccaggcccac cctgcagctc ccgctggcca acgatggggg cagccgctcg ccatcctcag 241 agagctcccc gcagcacccc acgccccccg cccggccccg ccacatgctg gggctcccgt 301 caaccctgtt cacaccccgc agcatggaga gcattgagat tgaccagaag ctgcaggaga 361 tcatgaagca gacgggctac ctgaccatcg ggggccagcg ctaccaggca gaaatcaacg 421 acctggagaa cttgggcgag atgggcagcg gcacctgcgg ccaggtgtgg aagatgcgct 481 tccggaagac cggccacgtc attgccgtta agcaaatgcg gcgctccggg aacaaggagg 541 agaacaagcg catcctcatg gacctggatg tggtgctgaa gagccacgac tgcccctaca 601 tcgtgcagtg ctttgggacg ttcatcacca acacggatgt cttcatcgcc atggagctca 661 tgggcacctg cgctgagaag ctcaagaagc ggatgcaggg ccccatcccc gagcgcattc 721 tgggcaagat gacagtggcg attgtgaagg cgctgtacta cctgaaggag aagcacggtg 781 tcatccaccg cgacgtcaag ccctccaaca tcctgctgga cgagcggggc cagatcaagc 841 tctgcgactt cggcatcagc ggccgcctgg tggactccaa agccaagacg cggagcgccg 901 gctgtgccgc ctacatggca cccgagcgca ttgacccccc agaccccacc aagccggact 961 atgacatccg ggccgacgta tggagcctgg gcatctcgtt gccctgcccg tctcccTCCC 1021 AGGTGGAGCT GGCAACAGGA CAGTTTCCCT ACAAGAACTG CAAGACGGAC TTTGAGGTCC 1081 TCACCAAAGT CCTACAGGAA GAGCCCCCGC TTCTGCCCGG ACACATGGGC TTCTCGGGGG 1141 ACTTCCAGTC CTTCGTCAAA GACTGCCTTA CTAAAGATCA CAGGAAGAGA CCAAAGTATA 1201 ATAAGCTACT TGAACACAGC TTCATCAAGC GCTACGAGAC GCTGGAGGTG GACGTGGCGT 1261 CCTGGTTCAA GGATGTCATG GCGAAGACTG AGTCACCGCG GACTAGCGGC GTCCTGAGCC 1321 AGCCCCACCT GCCCTTCCTC AGGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1381 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1441 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CAAAGCTAGG 1501 TTCCCCTATA TCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1561 att