Construct: ORF TRCN0000479781
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011928.1_s317c1
- Derived from:
- ccsbBroadEn_09983
- DNA Barcode:
- CACAGTAAACATGCCACTCCCAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF454 (285676)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479781
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285676 | ZNF454 | zinc finger protein 454 | NM_001178089.3 | 99.9% | 99.8% | 497A>C |
| 2 | human | 285676 | ZNF454 | zinc finger protein 454 | NM_001178090.3 | 99.9% | 99.8% | 497A>C |
| 3 | human | 285676 | ZNF454 | zinc finger protein 454 | NM_001323306.2 | 99.9% | 99.8% | 497A>C |
| 4 | human | 285676 | ZNF454 | zinc finger protein 454 | NM_182594.4 | 99.9% | 99.8% | 497A>C |
| 5 | human | 285676 | ZNF454 | zinc finger protein 454 | NM_001323307.2 | 24.3% | 18.3% | (many diffs) |
| 6 | human | 285676 | ZNF454 | zinc finger protein 454 | NM_001323308.2 | 24.3% | 18.3% | (many diffs) |
| 7 | human | 285676 | ZNF454 | zinc finger protein 454 | NM_001323309.2 | 24.3% | 18.3% | (many diffs) |
| 8 | human | 285676 | ZNF454 | zinc finger protein 454 | NM_001323310.2 | 24.3% | 18.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1635
- ORF length:
- 1566
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgtcagc cacctgccaa ccatggtcca ggaatcggtg accttcaagg 121 atgtggctat actgttcacc caggaagagt gggggcagct gagccccgcc cagagggccc 181 tgtacaggga cgtgatgctg gagaactaca gcaacctggt ctcactggga ctcttaggac 241 ccaaaccaga tacgttttcc cagctagaaa aaagggaagt gtggatgcca gaggacaccc 301 ctggaggctt ctgtcttgac tggatgacta tgcctgccag taagaaatct actgtcaagg 361 cagagattcc tgaagaagaa ttggatcaat ggacaataaa ggaaagattc agtagcagta 421 gtcactggaa gtgtgctagc ctgctggagt ggcaatgtgg aggccaggag atcagtttgc 481 agcgagtggt actcactcac cccaacaccc catcacagga atgtgatgaa tccgggagca 541 ctatgagctc atctcttcac agtgctcaaa gtcagggatt tcaacctagc aaaaatgcct 601 ttgagtgtag tgagtgtgga aaagtcttct ctaagagttc aactcttaat aaacatcaga 661 aaattcataa tgaaaaaaat gcaaatcaga aaattcatat taaggagaaa agatatgaat 721 gtagagaatg tgggaaagcc tttcaccaga gtacgcacct tatccatcac caaagaattc 781 acactggcga gaaaccctat gaatgtaagg aatgtggcaa ggccttctca gtgagctcct 841 cacttacgta ccatcagaaa attcatactg gagagaagcc ttttgaatgc aacttatgtg 901 gaaaagcttt tatccgaaat atacaccttg cccatcatca tagaatacat actggagaga 961 aaccttttaa atgtaacatt tgtgaaaaag cctttgtgtg cagggcacac cttaccaaac 1021 accagaatat ccacagtgga gagaaaccct ataaatgcaa tgaatgtgga aaagccttta 1081 atcagagtac aagtttcctt cagcatcaga gaattcacac tggagagaaa ccctttgaat 1141 gtaatgaatg tgggaaggcc ttcagggtga actcttccct tactgaacat cagagaattc 1201 atactggaga gaaaccttat aaatgtaatg aatgtgggaa agctttcagg gataattcaT 1261 CCTTTGCACG ACATCGGAAA ATTCACACTG GAGAGAAACC TTACAGATGT GGCTTGTGTG 1321 AGAAAGCCTT TCGGGACCAA TCAGCACTAG CCCAACATCA GAGAATTCAT ACTGGGGAAA 1381 AACCTTATAC ATGTAACATA TGTGAAAAAG CCTTCAGTGA CCATTCAGCC CTTACCCAAC 1441 ATAAGAGAAT TCATACTAGG GAAAAACCTT ACAAATGTAA AATCTGTGAG AAAGCCTTTA 1501 TCCGAAGCAC TCACCTGACT CAACATCAGA GGATTCACAC AGGAGAGAAA CCCTATAAAT 1561 GTAATAAATG TGGGAAAGCT TTTAACCAGA CTGCAAACCT CATTCAGCAT CAGAGACATC 1621 ATATTGGAGA GAAGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1681 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1741 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CACAGTAAAC ATGCCACTCC 1801 CAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt