Construct: ORF TRCN0000479799

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015685.1_s317c1
Derived from:
ccsbBroadEn_13908
DNA Barcode:
TCGGCGCGTGTTCTCAAAACTAGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MYL5 (4636)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479799
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4636 MYL5 myosin light chain 5 NM_001363650.2 99.7% 100% 243G>A
2 human 4636 MYL5 myosin light chain 5 NM_002477.1 76.1% 76.3% 1_123del;366G>A
3 human 4636 MYL5 myosin light chain 5 XM_017008245.1 76.1% 76.3% 1_123del;366G>A
4 human 4636 MYL5 myosin light chain 5 XM_017008246.1 76.1% 76.3% 1_123del;366G>A
5 human 4636 MYL5 myosin light chain 5 XM_006713886.2 40.1% 40.2% 1_588del;831G>A
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
462
ORF length:
396
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga tcagaaccga gatggcttca ttgacaagga ggacctgaag gacaccTATG
121CCTCCCTGGG CAAGACCAAC GTCAAGGACG ACGAGCTGGA CGCCATGCTC AAAGAGGCCT
181CGGGGCCCAT CAACTTCACC ATGTTTCTGA ACCTGTTTGG GGAGAAGCTG AGCGGTACCG
241ACGCCGAGGA GACCATTCTT AACGCCTTCA AGATGCTGGA CCCGGACGGG AAAGGGAAAA
301TCAACAAAGA GTACATCAAG CGTCTGCTGA TGTCCCAGGC TGACAAGATG ACGGCGGAAG
361AGGTGGACCA GATGTTCCAG TTCGCCTCCA TCGATGTGGC GGGCAACCTG GACTACAAGG
421CGCTCAGCTA CGTGATCACC CACGGGGAGG AGAAGGAGGA GTGCCCAACT TTCTTGTACA
481AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
541AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
601AGGACGATCG GCGCGTGTTC TCAAAACTAG CACGCGTTAA GTCgacaatc aacctctgga
661ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)

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