Construct: ORF TRCN0000479828
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016275.1_s317c1
- Derived from:
- ccsbBroadEn_16146
- DNA Barcode:
- TTAATTCGAGAGTACCCGGTTCGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ADGRD1 (283383)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479828
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283383 | ADGRD1 | adhesion G protein-coupled ... | NM_198827.5 | 99.4% | 88.1% | (many diffs) |
| 2 | human | 283383 | ADGRD1 | adhesion G protein-coupled ... | NM_001330497.2 | 95.9% | 85% | (many diffs) |
| 3 | human | 283383 | ADGRD1 | adhesion G protein-coupled ... | XM_005253566.2 | 92.6% | 81.3% | (many diffs) |
| 4 | human | 283383 | ADGRD1 | adhesion G protein-coupled ... | XM_011538204.2 | 92.5% | 88.3% | (many diffs) |
| 5 | human | 283383 | ADGRD1 | adhesion G protein-coupled ... | XM_011538205.2 | 89.3% | 78.4% | (many diffs) |
| 6 | human | 283383 | ADGRD1 | adhesion G protein-coupled ... | XM_011538206.2 | 87.9% | 77.1% | (many diffs) |
| 7 | human | 283383 | ADGRD1 | adhesion G protein-coupled ... | XM_011538207.2 | 83.8% | 90% | (many diffs) |
| 8 | human | 283383 | ADGRD1 | adhesion G protein-coupled ... | XM_011538208.2 | 79.9% | 69.1% | (many diffs) |
| 9 | human | 283383 | ADGRD1 | adhesion G protein-coupled ... | XM_011538209.2 | 77.4% | 66.6% | (many diffs) |
| 10 | human | 283383 | ADGRD1 | adhesion G protein-coupled ... | XM_011538210.2 | 65.3% | 66% | (many diffs) |
| 11 | human | 283383 | ADGRD1 | adhesion G protein-coupled ... | XM_011538211.2 | 63.9% | 50.7% | (many diffs) |
| 12 | human | 283383 | ADGRD1 | adhesion G protein-coupled ... | XM_011538212.2 | 56.1% | 44.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2496
- ORF length:
- 2427
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaaagctg ctgcggctgt gctgctggta ctcctggctg ctgctatttt 121 attacaactt tcaggtgcgt ggcgtctact ccagatcgca ggaccatcca ggatttcagg 181 tgttggcgtc tgcttcccat tactggccac tggagaatgt ggatgggatc catgaacttc 241 aggatacaac tggagatatt gtggaaggga aggtcaacaa aggcatttac ctgaaagagg 301 aaaagggagt cacgcttctc tattacggca ggtacaacag ctcctgcatc agcaagccag 361 agcagtgtgg ccctgaaggg gtcacgtttt cttttttctg gaagacacaa ggagaacagt 421 ctagaccaat cccttctgcg tatgggggac aggtcatctc caatgggttc aaagtctgct 481 ccagcggtgg cagaggctct gtggagctgt acacgcggga caattccatg acatgggagg 541 cctccttcag ccccccaggc ccctattgga ctcatgtcct atttacatgg aaatccaagg 601 agggcctgaa agtctacgtc aacgggaccc tgagcacctc tgatccgagt ggaaaagtgt 661 ctcgtgacta tggagagtcc aacgtcaacc tcgtgatagg gtctgagcag gaccaggcca 721 agtgttatga gaacggtgct ttcgatgagt tcatcatctg ggagcgggct ctgactccgg 781 atgagatcgc catgtacttc actgctgcca ttggaaagca tgctttattg tcttcaacgc 841 tgccaagcct cttcatgaca tccacagcaa gccccgtgat gcccacagat gcctaccatc 901 ccatcataac caacctgaca gaagagagaa aaaccttcca aagtcccgga gtgatactga 961 gttacctcca aaatgtatcc ctcagcttac ccagtaagtc cctctcggag cagacagcct 1021 tgaatctcac caagaccttc ttaaaagccg tgggagagat ccttctactg cctggttgga 1081 ttgctctgtc agaggacagc gccgtggtac tgagtctcat cgacactatt gacaccgtca 1141 tgggccatgt atcctccaac ctgcacggca gcacgcccca ggtcaccgtg gagggctcct 1201 ctgccatggc agagttttcc gtggccaaaa tcctgcccaa gaccgtgaat tcctcccatt 1261 accgcttccc ggcccacggg cagagcttca tccagatccc ccacgaggcc ttccacaggc 1321 acgcctggag caccgtcgtg ggtctgctgt accacagcat gcactactac ctgaacaaca 1381 tctggcccgc ccacaccaag atcgcggagg ccatgcatca ccaggactgc ctgctgttcg 1441 ccaccagcca cctgatttcc ctggaggtgt ccccaccacc caccctgtct cagaacctgt 1501 cgggctctcc actcattacg gtccacctca agcacagatt gacacgtaag cagcacagtg 1561 aggccaccaa cagcagcaac cgagtcttcg tgtactgcgc cttcctggac ttcagctccg 1621 gagaaggggt ctggtcgaac cacggctgtg cgctcacgag aggaaacctc acctactccg 1681 tctgccgctg cactcacctc accaactttg ccatcctcat gcaggtggtc ccgctggagc 1741 ttgcacgcgg acaccaggtg gcgctgtcgt ctatcagcta tgtgggctgc tccctctccg 1801 tgctctgcct ggtggccacg ctggtcacct tcgccgtgct gtcctccgtg agcaccatcc 1861 ggaaccagcg ctaccacatc cacgccaacc tgtccttcgc cgtgctggtg gcccaggtcc 1921 tgctgctcat tagtttccgc ctcgagccgg gcacgacccc ctgccaagtg atggccgtgc 1981 tcctacacta cttcttcctg agtgccttcg catggatgct ggtggagggg ctgcacctct 2041 acagcatggt gatcaaggtc tttgggtcgg aggacagcaa gcaccgttac tactatggga 2101 tgggatgggg ttttcctctt ctgatctgca tcatttcact gtcatttgcc atggacagtt 2161 acggaacaag caacaattgc tggctgtcgt tggcgagtgg cgccatctgg gcctttgtag 2221 cccctgctct gtttgtcatc gtggtcaaca ttggcatcct catcgctgtg accagagtca 2281 tctcacagat cagcgccgac aactacaaga tccatggaga ccccagtgcc ttcaagttga 2341 cagccaaGGC ACCATGGAAA AGCTGCTGCC CATCCTGGGT ACCTCGTGGG TCTTTGGCGT 2401 GCTTGCTGTC AACGGTTGTG CTGTGGTTTT CCAGTACATG TTTGCCACGC TCAACTCCCT 2461 GCAGGGACTG TTCATATTCC TCTTTCATTG TCTCCTGAAT TCAGAGGTGA GAGCCGCCTT 2521 CAAGCACAAA ACCAAGGTCT GGTCGCTCAC GAGCAGCTCC GCCCGCACCT CCAACGCGAA 2581 GCCCTTCCAC TCGGACCTCA TGAATGGGAC CCGACCAGGC ATGGCCTCCA CCAAGCTCAG 2641 CCCTTGGGAC AAGAGCAGCC ACTCTGCCCA CCGCGTCGAC CTGTCAGCCG TGTTGCCAAC 2701 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 2761 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 2821 ATCTTGTGGA AAGGACGATT AATTCGAGAG TACCCGGTTC GTACGCGTTA AGTCgacaat 2881 caacctctgg attacaaaat ttgtgaaaga tt