Construct: ORF TRCN0000479836
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004799.2_s317c1
- Derived from:
- ccsbBroadEn_11230
- DNA Barcode:
- ACTCTCGAAGTCTGCCGATTTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZSCAN20 (7579)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479836
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7579 | ZSCAN20 | zinc finger and SCAN domain... | XM_006710876.3 | 43.4% | 43.1% | (many diffs) |
| 2 | human | 7579 | ZSCAN20 | zinc finger and SCAN domain... | XM_005271171.3 | 41.2% | 41% | (many diffs) |
| 3 | human | 7579 | ZSCAN20 | zinc finger and SCAN domain... | XM_017002238.1 | 41.2% | 41% | (many diffs) |
| 4 | human | 7579 | ZSCAN20 | zinc finger and SCAN domain... | NM_145238.4 | 41.2% | 40.9% | (many diffs) |
| 5 | human | 7579 | ZSCAN20 | zinc finger and SCAN domain... | XM_006710874.3 | 41.2% | 40.9% | (many diffs) |
| 6 | human | 7579 | ZSCAN20 | zinc finger and SCAN domain... | XM_017002237.1 | 41.2% | 40.9% | (many diffs) |
| 7 | human | 7579 | ZSCAN20 | zinc finger and SCAN domain... | XM_017002239.1 | 16.8% | 16.5% | (many diffs) |
| 8 | human | 7579 | ZSCAN20 | zinc finger and SCAN domain... | XM_017002240.1 | 16.8% | 16.5% | (many diffs) |
| 9 | human | 7579 | ZSCAN20 | zinc finger and SCAN domain... | XM_017002241.1 | 16.8% | 16.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1365
- ORF length:
- 1299
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tatggccctg gaattgcaag cccaggcatc tccgcagcca gagcctgaag 121 aactcctgat tgtgaaactg gaagaggact cttggggatc agaatccaaa ctctgggaga 181 aggaccgtgg ctctgtctct ggcccagagg cctcccgcca gcgcttcagg caattccaat 241 acagggatgc agctggaccc cacgaggcct tcagccagct ctgggctctc tgctgtcgtt 301 ggctgaggcc ggagatccgt ctcaaagagc agatcctgga gctgctcgtg ctggagcagt 361 tcctgactat cttgcctagg gaggtccaga cctgggtgca ggcacgccac cctgagagtg 421 gtgaggaggc tgtggccttg gtggaggatt ggcaccgaga gaccaggact gcaggacagt 481 cgggactgga attgcataca gaagagacca ggcccttaaa gacaggggaa gaagctcaga 541 gcttccagct gcagccagtg gatccctggc ctgagggaca gtcccagaag aagggggtga 601 agaatacatg ccctgacctt cccaatcacc taaatgccga ggtggcacca cagcctttga 661 aagagagtgg agttccagtt tcaaaaccaa gtaatacctc cgagaaagag caaggaccag 721 agttttgggg tctaagtctt ataaattctg ggaaaaggag cactgcagat tacagcctgg 781 ataatgagcc agctcaggca ttgacctgga gggattcaag agcctgggag gaacaatacc 841 agtgggatgt ggaggacatg aaggtgtcag gtgttcactg gggctatgag gagaccaaga 901 ctttcctggc aattttgagt gaatctcctt tctctgaaaa gctccggact tgtcaccaga 961 accgccaggt atatcgggcc attgcagagc agctaagggc aaggggcttc ctgcggacac 1021 tggagcaatg tcgctatagg gtcaaaaacc tccTACGGAA TTACCGGAAA GCCAAGAGCA 1081 GCCACCCACC AGGTACCTGC CCCTTCTATG AGGAGCTGGA GGCCCTGGTC AGGGCTCGGA 1141 CAGCCATCAG AGCCACAGAT GGCCCAGGAG AGGCCGTGGC ACTTCCCAGG CTCGGGGATA 1201 GTGACGCAGA GATGGATGAG CAGGAGGAAG GGGGCTGGGA TCCTGAAGAA ATGGCAGAAG 1261 ACTGTAACGG TGCTGGCCTG GTCAATGTTG AGTCTACCCA GGGGCCCAGG ATTGCAGGGG 1321 CCCCAGCTCT GTTCCAGAGT CGTATTGGTA AGAATATGGG GGTTTACCCA ACTTTCTTGT 1381 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1441 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1501 GAAAGGACGA ACTCTCGAAG TCTGCCGATT TTTTACGCGT TAAGTCgaca atcaacctct 1561 ggattacaaa atttgtgaaa gatt