Construct: ORF TRCN0000479838
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000326.1_s317c1
- Derived from:
- ccsbBroadEn_03197
- DNA Barcode:
- TGGTCAGGATTAAAAAAGACTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFA13 (51079)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479838
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51079 | NDUFA13 | NADH:ubiquinone oxidoreduct... | NM_015965.7 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 498
- ORF length:
- 432
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgtcaaag gtgaagcagg acatgccTCC GCCGGGGGGC TATGGGCCCA 121 TCGACTACAA ACGGAACTTG CCGCGTCGAG GACTGTCGGG CTACAGCATG CTGGCCATAG 181 GGATTGGAAC CCTGATCTAC GGGCACTGGA GCATAATGAA GTGGAACCGT GAGCGCAGGC 241 GCCTACAAAT CGAGGACTTC GAGGCTCGCA TCGCGCTGTT GCCACTGTTA CAGGCAGAAA 301 CCGACCGGAG GACCTTGCAG ATGCTTCGGG AGAACCTGGA GGAGGAGGCC ATCATCATGA 361 AGGACGTGCC CGACTGGAAG GTGGGGGAGT CTGTGTTCCA CACAACCCGC TGGGTGCCCC 421 CCTTGATCGG GGAGCTGTAC GGGCTGCGCA CCACAGAGGA GGCTCTCCAT GCCAGCCACG 481 GCTTCATGTG GTACACGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 541 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 601 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGGTCAG GATTAAAAAA 661 GACTATTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt