Construct: ORF TRCN0000479840
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014025.1_s317c1
- Derived from:
- ccsbBroadEn_13025
- DNA Barcode:
- AAGCTCACCCGGTCACACACAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBA3FP (113691)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479840
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7278 | TUBA3C | tubulin alpha 3c | NM_006001.3 | 27.6% | 20.1% | (many diffs) |
| 2 | human | 113457 | TUBA3D | tubulin alpha 3d | NM_080386.4 | 27.4% | 20.1% | (many diffs) |
| 3 | human | 112714 | TUBA3E | tubulin alpha 3e | NM_207312.3 | 27.4% | 20.4% | (many diffs) |
| 4 | human | 113691 | TUBA3FP | tubulin alpha 3f pseudogene | NR_003608.1 | 23.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 528
- ORF length:
- 462
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gctgtcaggg aatggcctgg aggagcaggc ggcccagcac cttgatgaac 121 tcctgctggc ccacacagac ctgaagtccc tggaccTGAG CTACAACCAG CTGAATGACC 181 AAGCAGATCT GTGCATGGGA CTGCAGGGCT TCCTCATCTT CCACAGCTTT GGGGGCGGCA 241 CTGGCTCTGG GTTCGTGTCT CTGCTCATGA AGCGGCTCTC GGTGGACTAC GGGAAGAAGT 301 CCAAGCTGGA GTTTGCCATT TGCCCAGCCC CCCAGGTCTC CATGGCCATG ACGGAGCCCT 361 ACAACTCCAT CCTGACCACC TACACGACCC TGGAACATTC TGACTGTGCC TTCATAGTCA 421 ACAGCAAAGC CACCTATGAC ATGTCAGCAC AACCTGGACA TCGAGTGTCC CACGTACACC 481 AACCTCAGTC GTCTGGGCAG ATCGTGTCCT CCATCACGGC CTCCTTGTGC CCAACTTTCT 541 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 601 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 661 GTGGAAAGGA CGAAAGCTCA CCCGGTCACA CACAATAACG CGTTAAGTCg acaatcaacc 721 tctggattac aaaatttgtg aaagatt