Construct: ORF TRCN0000479851

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003834.2_s317c1
Derived from:
ccsbBroadEn_08605
DNA Barcode:
TGCGTGCACTAAATATATCAGCCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PBK (55872)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479851
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55872 PBK PDZ binding kinase NM_001363040.2 99.8% 100% 99G>A
2 human 55872 PBK PDZ binding kinase NM_018492.4 99.8% 100% 99G>A
3 human 55872 PBK PDZ binding kinase NM_001278945.2 96.5% 96.6% 99G>A;595_627del
4 mouse 52033 Pbk PDZ binding kinase NM_023209.2 84.7% 85.4% (many diffs)
5 mouse 52033 Pbk PDZ binding kinase XM_006519257.3 84.7% 85.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1032
ORF length:
966
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga agggatcagt aatttcaaga caccaagcaa attatcagaa aaaaagaaat
121ctgtattatg ttcaactcca actataaata tcccggcctc tccatttatg cagaagcttg
181gctttggtac tggggtaaat gtgtacctaa tgaaaagatc tccaagaggt ttgtctcatt
241ctccttgggc tgtaaaaaag attaatccta tatgtaatga tcattatcga agtgtgtatc
301aaaagagact aatggatgaa gctaagattt tgaaaagcct tcatcatcca aacattgttg
361gttatcgtgc ttttactgaa gccaatgatg gcagtctgtg tcttgctatg gaatatggag
421gtgaaaagtc tctaaatgac ttaatagaag aacgatataa agccagccaa gatccttttc
481cagcagccat aattttaaaa gttgctttga atatggcaag agggttaaag tatctgcacc
541aagaaaagaa actgcttcat ggagacataa agtcttcaaa tgttgtaatt aaaggcgatt
601ttgaaacaat taaaatctgt gatgtaggag tctctctacc actGGATGAA AATATGACTG
661TGACTGACCC TGAGGCTTGT TACATTGGCA CAGAGCCATG GAAACCCAAA GAAGCTGTGG
721AGGAGAATGG TGTTATTACT GACAAGGCAG ACATATTTGC CTTTGGCCTT ACTTTGTGGG
781AAATGATGAC TTTATCGATT CCACACATTA ATCTTTCAAA TGATGATGAT GATGAAGATA
841AAACTTTTGA TGAAAGTGAT TTTGATGATG AAGCATACTA TGCAGCGTTG GGAACTAGGC
901CACCTATTAA TATGGAAGAA CTGGATGAAT CATACCAGAA AGTAATTGAA CTCTTCTCTG
961TATGCACTAA TGAAGACCCT AAAGATCGTC CTTCTGCTGC ACACATTGTT GAAGCTCTGG
1021AAACAGATGT CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1081ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1141CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGC GTGCACTAAA TATATCAGCC
1201GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)