Transcript: Mouse NM_023209.2

Mus musculus PDZ binding kinase (Pbk), mRNA.

Source:
NCBI, updated 2017-06-25
Taxon:
Mus musculus (mouse)
Gene:
Pbk (52033)
Length:
1663
CDS:
245..1237

Additional Resources:

NCBI RefSeq record:
NM_023209.2
NBCI Gene record:
Pbk (52033)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_023209.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000322234 TGGCCAATGTTGTGGTCTAAG pLKO_005 1204 CDS 100% 10.800 15.120 N Pbk n/a
2 TRCN0000222270 GTCAGCGTTTACCTAATGAAA pLKO.1 371 CDS 100% 5.625 7.875 N Pbk n/a
3 TRCN0000361714 ACGTACATGTGGTACAGATAT pLKO_005 1420 3UTR 100% 13.200 10.560 N Pbk n/a
4 TRCN0000322169 TAGATTCTAGAAGTAGCTTTA pLKO_005 1291 3UTR 100% 10.800 7.560 N Pbk n/a
5 TRCN0000322233 TCTTCTGTGTGTGCACTAATG pLKO_005 1128 CDS 100% 10.800 7.560 N Pbk n/a
6 TRCN0000222273 CCTTACTCTGTGGGAAATGAT pLKO.1 943 CDS 100% 5.625 3.938 N Pbk n/a
7 TRCN0000322231 CCTTACTCTGTGGGAAATGAT pLKO_005 943 CDS 100% 5.625 3.938 N Pbk n/a
8 TRCN0000027585 CCAGATGATGATGTTGATGAA pLKO.1 992 CDS 100% 4.950 3.465 N LOC279333 n/a
9 TRCN0000322232 AGGCCTGTTATATTGGTACTG pLKO_005 849 CDS 100% 4.050 2.835 N Pbk n/a
10 TRCN0000026688 CCACACGTCAATCTTCCAGAT pLKO.1 977 CDS 100% 4.050 2.835 N Pbk n/a
11 TRCN0000222271 GCTATGGAGTATGGAGGTGAA pLKO.1 581 CDS 100% 4.050 2.835 N Pbk n/a
12 TRCN0000361724 TGCTGCACACATCGTTGAAGC pLKO_005 1171 CDS 100% 4.050 2.835 N Pbk n/a
13 TRCN0000222272 AGAGACTAACTGATGAAGCTA pLKO.1 480 CDS 100% 3.000 2.100 N Pbk n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_023209.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08605 pDONR223 100% 84.7% 85.4% None (many diffs) n/a
2 ccsbBroad304_08605 pLX_304 0% 84.7% 85.4% V5 (many diffs) n/a
3 TRCN0000479851 TGCGTGCACTAAATATATCAGCCG pLX_317 40.4% 84.7% 85.4% V5 (many diffs) n/a
4 ccsbBroadEn_15110 pDONR223 0% 84.7% 85.4% None (many diffs) n/a
5 ccsbBroad304_15110 pLX_304 0% 84.7% 85.4% V5 (many diffs) n/a
6 TRCN0000480509 TCCCTGGCGTCGGGGTCAATTTTC pLX_317 37.7% 84.7% 85.4% V5 (many diffs) n/a
7 TRCN0000488975 TAATCCACATTTACTCCCAACTTA pLX_317 37.5% 84.7% 85.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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