Construct: ORF TRCN0000479869
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011287.1_s317c1
- Derived from:
- ccsbBroadEn_07011
- DNA Barcode:
- CGAACCATTATCGCCGATTCCGAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SULT1E1 (6783)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479869
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6783 | SULT1E1 | sulfotransferase family 1E ... | NM_005420.3 | 99.7% | 2.3% | 24T>A;460T>C |
| 2 | human | 6783 | SULT1E1 | sulfotransferase family 1E ... | XM_011532210.2 | 56.3% | 3.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 87
- ORF length:
- 21
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ttctgaactt gactattaag aaaagtttga agaagtccat gggattctaa 121 tgtataaaga ttttgtcaaa tattgggata atgtggaagc gttccaggca agaccagatg 181 atcttgtcat tgccacctac cctaaatctg gtacaacctg ggttagtgaa attgtgtata 241 tgatctataa agagggtgat gtggaaaagt gcaaagaaga tgtaattttt aatcgaatac 301 ctttcctgga atgcagaaaa gaaaacctca tgaatggagt aaaacaatta gatgagatga 361 attctcctag aattgtgaag actcatttgc cacctgaact tcttcctgcc tcattttggg 421 aaaaggattg taagataatc tatctttgcc ggaatgcaaa ggatgtggct gtttcctttt 481 attatttctt tctaatggtg gctggtcatc caaatcctgg atcccTTCCA GAGTTTGTGG 541 AGAAATTCAT GCAAGGACAG GTTCCTTATG GTTCCTGGTA TAAACATGTA AAATCTTGGT 601 GGGAAAAGGG AAAGAGTCCA CGTGTACTAT TTCTTTTCTA CGAAGACCTG AAAGAGGATA 661 TCAGAAAAGA GGTGATAAAA TTGATACATT TCCTGGAAAG GAAGCCATCA GAGGAGCTTG 721 TGGACAGGAT TATACATCAT ACTTCGTTCC AAGAGATGAA GAACAATCCA TCCACAAATT 781 ACACAACACT GCCAGACGAA ATTATGAACC AGAAATTGTC GCCCTTCATG AGAAAGGGAA 841 TTACAGGAGA CTGGAAAAAT CACTTTACAG TAGCCCTGAA TGAAAAATTT GATAAACATT 901 ATGAGCAGCA AATGAAGGAA TCTACACTGA AGTTTCGAAC TGAGATCTAC CCAACTTTCT 961 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1021 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1081 GTGGAAAGGA CGACGAACCA TTATCGCCGA TTCCGAAACG CGTTAAGTCg acaatcaacc 1141 tctggattac aaaatttgtg aaagatt