Construct: ORF TRCN0000479872

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006930.1_s317c1
Derived from:
ccsbBroadEn_05718
DNA Barcode:
ATCGCTTTTACCTCCGCAGCCCTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SYCE3 (644186)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479872
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 644186 SYCE3 synaptonemal complex centra... NM_001123225.3 100% 100%
2 human 644186 SYCE3 synaptonemal complex centra... XM_024452261.1 100% 100%
3 mouse 75459 Syce3 synaptonemal complex centra... NM_001162880.1 87.1% 89.7% (many diffs)
4 mouse 75459 Syce3 synaptonemal complex centra... NM_001162882.1 87.1% 89.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
93
ORF end:
357
ORF length:
264
Sequence:
1TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT
61GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGATGA TGCTGACCCT GAGGAAAGAA
121ACTATGACAA CATGCTGAAA ATGCTGTCAG ATCTGAATAA GGACTTGGAA AAGCTATTAG
181AAGAGATGGA GAAAATCTCA GTGCAGGCGA CCTGGATGGC CTATGACATG GTGGTGATGC
241GCACCAACCC TACGCTGGCC GAGTCCATGC GTCGGCTGGA GGATGCCTTC GTCAACTGCA
301AGGAGGAGAT GGAGAAGAAC TGGCAAGAGC TGCTGCATGA GACCAAGCAA AGGCTGTTGC
361CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
421TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
481ATATATCTTG TGGAAAGGAC GAATCGCTTT TACCTCCGCA GCCCTCACGC GTTAAGTCga
541caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)