Construct: ORF TRCN0000479872
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006930.1_s317c1
- Derived from:
- ccsbBroadEn_05718
- DNA Barcode:
- ATCGCTTTTACCTCCGCAGCCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYCE3 (644186)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479872
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 644186 | SYCE3 | synaptonemal complex centra... | NM_001123225.3 | 100% | 100% | |
| 2 | human | 644186 | SYCE3 | synaptonemal complex centra... | XM_024452261.1 | 100% | 100% | |
| 3 | mouse | 75459 | Syce3 | synaptonemal complex centra... | NM_001162880.1 | 87.1% | 89.7% | (many diffs) |
| 4 | mouse | 75459 | Syce3 | synaptonemal complex centra... | NM_001162882.1 | 87.1% | 89.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 357
- ORF length:
- 264
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGATGA TGCTGACCCT GAGGAAAGAA 121 ACTATGACAA CATGCTGAAA ATGCTGTCAG ATCTGAATAA GGACTTGGAA AAGCTATTAG 181 AAGAGATGGA GAAAATCTCA GTGCAGGCGA CCTGGATGGC CTATGACATG GTGGTGATGC 241 GCACCAACCC TACGCTGGCC GAGTCCATGC GTCGGCTGGA GGATGCCTTC GTCAACTGCA 301 AGGAGGAGAT GGAGAAGAAC TGGCAAGAGC TGCTGCATGA GACCAAGCAA AGGCTGTTGC 361 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 421 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 481 ATATATCTTG TGGAAAGGAC GAATCGCTTT TACCTCCGCA GCCCTCACGC GTTAAGTCga 541 caatcaacct ctggattaca aaatttgtga aagatt