Construct: ORF TRCN0000479904
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007810.2_s317c1
- Derived from:
- ccsbBroadEn_14998
- DNA Barcode:
- CACTCGAGGCGGCTCCCTGGCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FASTKD2 (22868)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479904
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22868 | FASTKD2 | FAST kinase domains 2 | NM_001136193.2 | 100% | 100% | |
| 2 | human | 22868 | FASTKD2 | FAST kinase domains 2 | NM_001136194.1 | 100% | 100% | |
| 3 | human | 22868 | FASTKD2 | FAST kinase domains 2 | NM_014929.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2196
- ORF length:
- 2130
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt gacaactttg aagccatttg gaagtgtttc agtggagagc aaaatgaata 121 acaaagcggg ctcctttttc tggaacctta gacaattcag tacattagtt tcaacaagca 181 gaactatgag gctatgttgt ttgggacttt gcaaaccaaa aatagttcat tcaaactgga 241 acattttaaa taactttcat aacagaatgc aatcaactga tatcattaga tatctctttc 301 aggatgcatt catttttaaa tcagatgttg gctttcaaac aaagggcata agcactctaa 361 cagcccttag aattgaaaga ctactttatg ctaaaagact gttttttgac tcaaagcagt 421 ctcttgtccc tgttgataaa tctgatgatg aattgaagaa agtaaacctt aatcatgaag 481 tctccaatga agatgttctt accaaggaaa caaaaccaaa ccgtatcagc agtagaaaac 541 tgtctgagga atgtaattcc ctgagtgatg tgttagatgc attttcaaaa gcgcccacat 601 ttcctagtag caactatttc acagcaatgt ggacaattgc caaaagactg tctgatgacc 661 agaagcgctt tgaaaaacga ctgatgttta gccaccctgc atttaatcag ctctgtgaac 721 atatgatgag agaagccaag atcatgcagt ataagtacct actgttcagt cttcacgcca 781 tagtgaagct tggaatccct cagaacacta ttttggtgca gactttgctg agggtgaccc 841 aggaacgtat caatgagtgt gatgagatat gcctttcagt tttgtcaact gttttagagg 901 caatggaacc atgcaagaat gttcatgttc tacgaacggg attcagaata ctagttgatc 961 agcaagtttg gaaaatagaa gatgtcttca cattacaagt tgtgatgaag tgtattggaa 1021 aagatgcacc gattgctctt aagaggaaac tggagatgaa agccttgagg gaattagaca 1081 gattttctgt tttgaatagc caacacatgt ttgaagtact agctgccatg aatcaccgat 1141 ctcttatact cctggatgaa tgcagtaagg tggtcctaga taatatccat gggtgtcctt 1201 taagaataat gatcaacata ttgcagtcct gcaaagacct ccagtaccat aatttggatc 1261 tcttcaaggg acttgcagat tatgtggctg caactttcga catctggaag ttcagaaaag 1321 ttctttttat cctcatttta tttgaaaacc ttggctttcg acctgttggt ttaatggacc 1381 tgtttatgaa gagaatagta gaggatcctg aatccctaaa catgaaaaac attctatcta 1441 ttcttcatac ttactcttct ctcaatcatg tctacaaatg ccagaacaaa gaacagttcg 1501 tggaagttat ggctagtgct ctgactggtt atcttcacac tatttcttct gaaaacttat 1561 tggatgcagt atattcattt tgcttgatga attactttcc cctggctcct tttaatcagc 1621 ttctgcaaaa agacatcatc agtgagctgc tgacatcaga tgacatgaag aatgcttaca 1681 agctgcatac tttggatact tgtctaaaac ttgatgatac tgtctatctg agggacatag 1741 ccttgtcact cccacagctg ccgcgggagc tgccatcgtc acatacaaat gcaaaggtgg 1801 cagaggtgct gagcagccTT CTGGGAGGTG AAGGACACTT CTCAAAGGAT GTGCACTTGC 1861 CACACAATTA TCATATTGAT TTTGAAATCA GAATGGACAC TAACAGGAAT CAAGTGCTAC 1921 CACTTTCTGA TGTGGATACA ACTTCTGCTA CAGATATTCA AAGAGTAGCT GTGCTATGTG 1981 TTTCCAGATC TGCTTATTGT TTGGGTTCAA GCCACCCCAG AGGATTCCTT GCTATGAAAA 2041 TGCGGCATTT GAATGCAATG GGTTTTCATG TGATCTTGGT CAATAACTGG GAGATGGACA 2101 AACTAGAGAT GGAAGATGCA GTCACATTTT TGAAGACTAA AATCTATTCA GTAGAAGCTC 2161 TTCCTGTTGC TGCTGTAAAT GTGCAAAGCA CACAATACCC AACTTTCTTG TACAAAGTGG 2221 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2281 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2341 ACACTCGAGG CGGCTCCCTG GCCTCACGCG TTAAGTCgac aatcaacctc tggattacaa 2401 aatttgtgaa agatt