Construct: ORF TRCN0000479908
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002923.1_s317c1
- Derived from:
- ccsbBroadEn_13909
- DNA Barcode:
- ACCTGCGGAAAATTATTTTGGATC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFA2 (4695)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479908
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4695 | NDUFA2 | NADH:ubiquinone oxidoreduct... | NM_002488.4 | 99.6% | 98.9% | 291delT |
| 2 | human | 4695 | NDUFA2 | NADH:ubiquinone oxidoreduct... | NM_001185012.1 | 74.1% | 70.7% | (many diffs) |
| 3 | human | 4695 | NDUFA2 | NADH:ubiquinone oxidoreduct... | NR_033697.1 | 34.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 363
- ORF length:
- 297
- Sequence:
-
1 tcttccattt CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGC GGCGGCCGCA GCAAGTCGAG GAGTCGGGGC AAAGCTGGGC CTGCGTGAGA 121 TTCGCATCCA CTTATGTCAG CGCTCGCCCG GCAGCCAGGG CGTCAGGGAC TTCATTGAGA 181 AACGCTACGT GGAGCTGAAG AAGGCGAATC CCGACCTACC CATCCTAATC CGCGAATGCT 241 CCGATGTGCA GCCCAAGCTC TGGGCCCGCT ACGCATTTGG CCAAGAGACG AATGTCCCTT 301 TGAACAACTT CAGTGCTGAT CAGGTAACCA GAGCCCTGGA GAACGTTCTA AGTGGAAAGC 361 CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT 421 CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG 481 GCTTTATATA TCTTGTGGAA AGGACGAACC TGCGGAAAAT TATTTTGGAT CACGCGTTAA 541 GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t