Construct: ORF TRCN0000479910

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000073.1_s317c1
Derived from:
ccsbBroadEn_15383
DNA Barcode:
TTACACGATAACCCCGACATACGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHAT (1103)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479910
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1103 CHAT choline O-acetyltransferase NM_001142929.1 99.9% 100% 1287T>C
2 human 1103 CHAT choline O-acetyltransferase NM_001142934.1 99.9% 100% 1287T>C
3 human 1103 CHAT choline O-acetyltransferase NM_020984.3 99.9% 100% 1287T>C
4 human 1103 CHAT choline O-acetyltransferase NM_020985.3 99.9% 100% 1287T>C
5 human 1103 CHAT choline O-acetyltransferase NM_020986.3 99.9% 100% 1287T>C
6 human 1103 CHAT choline O-acetyltransferase NM_001142933.1 94.5% 94.5% 1_108del;1395T>C
7 human 1103 CHAT choline O-acetyltransferase NM_020549.4 84.1% 84.2% 1_354del;1641T>C
8 mouse 12647 Chat choline acetyltransferase NM_009891.2 84.3% 85.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1959
ORF length:
1890
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcagcaaaa actcccagca gtgaggagtc tgggctgccc aaactgcccg
121tgcccccgct gcagcagacc ctggccacgt acctgcagtg catgcgacac ttggtgtctg
181aggagcagtt caggaagagc caggccattg tgcagcagtt tggggcccct ggtggcctcg
241gcgagaccct gcagcagaaa ctcctggagc ggcaggagaa gacagccaac tgggtgtctg
301agtactggct gaatgacatg tatctcaaca accgcctggc cctgcctgtc aactccagcc
361ctgccgtgat ctttgctcgg cagcacttcc ctggcaccga tgaccagctg aggtttgcag
421ccagcctcat ctctggtgta ctcagctaca aggccctgct ggacagccac tccattccca
481ctgactgtgc caaaggccag ctgtcagggc agcccctttg catgaagcaa tactatgggc
541tcttctcctc ctaccggctc cccggccata cccaggacac gctggtggct cagaacagca
601gcatcatgcc ggagcctgag cacgtcatcg tagcctgctg caatcagttc tttgtcttgg
661atgttgtcat taatttccgc cgtctcagtg agggggatct gttcactcag ttgagaaaga
721tagtcaaaat ggcttccaac gaggacgagc gtttgcctcc aattggcctg ctgacgtctg
781acgggaggag cgagtgggcc gaggccagga cggtcctcgt gaaagactcc accaaccggg
841actcgctgga catgattgag cgctgcatct gccttgtatg cctggacgcg ccaggaggcg
901tggagctcag cgacacccac agggcactcc agctccttca cggcggaggc tacagcaaga
961acggggccaa tcgctggtac gacaagtccc tgcagtttgt ggtgggccga gacggcacct
1021gcggtgtggt gtgcgaacac tccccattcg atggcatcgt cctggtgcag tgcactgagc
1081atctgctcaa gcacatgacg cagagcagca ggaagctgat ccgagcagac tccgtcagcg
1141agctccccgc cccccggagg ctgcggtgga aatgctcccc ggaaattcaa ggccacttag
1201cctcctcggc agaaaaactt caacgaatag taaagaacct tgacttcatt gtctataagt
1261ttgacaacta tgggaaaaca ttcattaaga agcagaaatg cagccctgat gccttcatcc
1321aggtggccct ccagctggcc ttctacaggc tccaccgaag actggtgccc acctacgaga
1381gcgcgtccat ccgccgattc caggagggac gcgtggacaa catcagatcg gccactccag
1441aggcactggc ttttgtgaga gccgtgactg accacaaggc tgctgtgcca gcttctgaga
1501agcttctgct cctgaaggat gccatccgtg cccagactgc atacacagtc atggccataa
1561cagggatggc cattgacaac cacctgctgg cactgcggga gctggcccgg gCCATGTGCA
1621AGGAGCTGCC CGAGATGTTC ATGGATGAAA CCTACCTGAT GAGCAACCGG TTTGTCCTCT
1681CCACTAGCCA GGTGCCCACA ACCACGGAGA TGTTCTGCTG CTATGGTCCT GTGGTCCCAA
1741ATGGGTATGG TGCCTGCTAC AACCCCCAGC CAGAGACCAT CCTTTTCTGC ATCTCTAGCT
1801TTCACAGCTG CAAAGAGACT TCTTCTAGCA AGTTTGCAAA AGCTGTGGAA GAAAGCCTCA
1861TTGACATGAG AGACCTCTGC AGTCTGCTGC CGCCTACTGA GAGCAAGCCA TTGGCAACAA
1921AGGAAAAAGC CACGAGGCCC AGCCAGGGAC ACCAACCTTT GCCAACTTTC TTGTACAAAG
1981TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
2041GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
2101ACGATTACAC GATAACCCCG ACATACGCAC GCGTTAAGTC gacaatcaac ctctggatta
2161caaaatttgt gaaagatt

Download FASTA (ORF) (Full)

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