Construct: ORF TRCN0000479910
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000073.1_s317c1
- Derived from:
- ccsbBroadEn_15383
- DNA Barcode:
- TTACACGATAACCCCGACATACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHAT (1103)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479910
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1103 | CHAT | choline O-acetyltransferase | NM_001142929.1 | 99.9% | 100% | 1287T>C |
| 2 | human | 1103 | CHAT | choline O-acetyltransferase | NM_001142934.1 | 99.9% | 100% | 1287T>C |
| 3 | human | 1103 | CHAT | choline O-acetyltransferase | NM_020984.3 | 99.9% | 100% | 1287T>C |
| 4 | human | 1103 | CHAT | choline O-acetyltransferase | NM_020985.3 | 99.9% | 100% | 1287T>C |
| 5 | human | 1103 | CHAT | choline O-acetyltransferase | NM_020986.3 | 99.9% | 100% | 1287T>C |
| 6 | human | 1103 | CHAT | choline O-acetyltransferase | NM_001142933.1 | 94.5% | 94.5% | 1_108del;1395T>C |
| 7 | human | 1103 | CHAT | choline O-acetyltransferase | NM_020549.4 | 84.1% | 84.2% | 1_354del;1641T>C |
| 8 | mouse | 12647 | Chat | choline acetyltransferase | NM_009891.2 | 84.3% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1959
- ORF length:
- 1890
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagcaaaa actcccagca gtgaggagtc tgggctgccc aaactgcccg 121 tgcccccgct gcagcagacc ctggccacgt acctgcagtg catgcgacac ttggtgtctg 181 aggagcagtt caggaagagc caggccattg tgcagcagtt tggggcccct ggtggcctcg 241 gcgagaccct gcagcagaaa ctcctggagc ggcaggagaa gacagccaac tgggtgtctg 301 agtactggct gaatgacatg tatctcaaca accgcctggc cctgcctgtc aactccagcc 361 ctgccgtgat ctttgctcgg cagcacttcc ctggcaccga tgaccagctg aggtttgcag 421 ccagcctcat ctctggtgta ctcagctaca aggccctgct ggacagccac tccattccca 481 ctgactgtgc caaaggccag ctgtcagggc agcccctttg catgaagcaa tactatgggc 541 tcttctcctc ctaccggctc cccggccata cccaggacac gctggtggct cagaacagca 601 gcatcatgcc ggagcctgag cacgtcatcg tagcctgctg caatcagttc tttgtcttgg 661 atgttgtcat taatttccgc cgtctcagtg agggggatct gttcactcag ttgagaaaga 721 tagtcaaaat ggcttccaac gaggacgagc gtttgcctcc aattggcctg ctgacgtctg 781 acgggaggag cgagtgggcc gaggccagga cggtcctcgt gaaagactcc accaaccggg 841 actcgctgga catgattgag cgctgcatct gccttgtatg cctggacgcg ccaggaggcg 901 tggagctcag cgacacccac agggcactcc agctccttca cggcggaggc tacagcaaga 961 acggggccaa tcgctggtac gacaagtccc tgcagtttgt ggtgggccga gacggcacct 1021 gcggtgtggt gtgcgaacac tccccattcg atggcatcgt cctggtgcag tgcactgagc 1081 atctgctcaa gcacatgacg cagagcagca ggaagctgat ccgagcagac tccgtcagcg 1141 agctccccgc cccccggagg ctgcggtgga aatgctcccc ggaaattcaa ggccacttag 1201 cctcctcggc agaaaaactt caacgaatag taaagaacct tgacttcatt gtctataagt 1261 ttgacaacta tgggaaaaca ttcattaaga agcagaaatg cagccctgat gccttcatcc 1321 aggtggccct ccagctggcc ttctacaggc tccaccgaag actggtgccc acctacgaga 1381 gcgcgtccat ccgccgattc caggagggac gcgtggacaa catcagatcg gccactccag 1441 aggcactggc ttttgtgaga gccgtgactg accacaaggc tgctgtgcca gcttctgaga 1501 agcttctgct cctgaaggat gccatccgtg cccagactgc atacacagtc atggccataa 1561 cagggatggc cattgacaac cacctgctgg cactgcggga gctggcccgg gCCATGTGCA 1621 AGGAGCTGCC CGAGATGTTC ATGGATGAAA CCTACCTGAT GAGCAACCGG TTTGTCCTCT 1681 CCACTAGCCA GGTGCCCACA ACCACGGAGA TGTTCTGCTG CTATGGTCCT GTGGTCCCAA 1741 ATGGGTATGG TGCCTGCTAC AACCCCCAGC CAGAGACCAT CCTTTTCTGC ATCTCTAGCT 1801 TTCACAGCTG CAAAGAGACT TCTTCTAGCA AGTTTGCAAA AGCTGTGGAA GAAAGCCTCA 1861 TTGACATGAG AGACCTCTGC AGTCTGCTGC CGCCTACTGA GAGCAAGCCA TTGGCAACAA 1921 AGGAAAAAGC CACGAGGCCC AGCCAGGGAC ACCAACCTTT GCCAACTTTC TTGTACAAAG 1981 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2041 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2101 ACGATTACAC GATAACCCCG ACATACGCAC GCGTTAAGTC gacaatcaac ctctggatta 2161 caaaatttgt gaaagatt