Construct: ORF TRCN0000479911
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016298.1_s317c1
- Derived from:
- ccsbBroadEn_13353
- DNA Barcode:
- GATCACCTCGCTCCAGTCACGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IDO2 (169355)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479911
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 169355 | IDO2 | indoleamine 2,3-dioxygenase 2 | NM_194294.2 | 39.8% | 39% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 585
- ORF length:
- 516
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttgcatttt cattattatg atacttcaaa caaaataatg gagccccaca 121 gaccgaatgt gaagacagca gtgccattgt ctttggaaag ctatcacata tctgaagagt 181 atggctTTCT TCTTCCAGAT TCTCTGAAAG AACTTCCAGA TCATTATAGG CCTTGGATGG 241 AAATTGCCAA CAAACTTCCT CAATTGATTG ATGCTCACCA GCTTCAAGCT CATGTGGACA 301 AGATGCCCCT GCTGAGCTGC CAGTTCCTGA AGGGTCACCG GGAGCAGCGC CTGGCCCACC 361 TGGTCCTGAG CTTCCTCACC ATGGGTTATG TCTGGCAGGA AGGAGAGGCG CAGCCTGCAG 421 AGGTCCTGCC AAGGAATCTT GCCCTTCCAT TTGTCGAAGT CTCCAGGAAC TTGGGGCTCC 481 CTCCTATCCT GGTCCACTCA GACTTGGTGC TGACGAACTG GACCAAAAAA GATCCAGACG 541 GAGACGGAGT CTCCCTCTGT CTCCCAGGCT GGAGTGCAGT GGCATTGCCA ACTTTCTTGT 601 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 661 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 721 GAAAGGACGA GATCACCTCG CTCCAGTCAC GTTTACGCGT TAAGTCgaca atcaacctct 781 ggattacaaa atttgtgaaa gatt