Construct: ORF TRCN0000479913
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014430.1_s317c1
- Derived from:
- ccsbBroadEn_07604
- DNA Barcode:
- CGTGACAAATGCGGATTCCTGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFDP1 (10428)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479913
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10428 | CFDP1 | craniofacial development pr... | NM_006324.3 | 99.7% | 99.6% | 834T>C;885C>A |
| 2 | human | 10428 | CFDP1 | craniofacial development pr... | XM_011522814.2 | 74.2% | 72.9% | (many diffs) |
| 3 | human | 10428 | CFDP1 | craniofacial development pr... | XM_011522815.2 | 73.2% | 72.5% | (many diffs) |
| 4 | human | 10428 | CFDP1 | craniofacial development pr... | XR_001751819.2 | 64.2% | (many diffs) | |
| 5 | human | 10428 | CFDP1 | craniofacial development pr... | XR_933183.2 | 62.6% | (many diffs) | |
| 6 | human | 10428 | CFDP1 | craniofacial development pr... | XR_933182.3 | 61.8% | (many diffs) | |
| 7 | human | 10428 | CFDP1 | craniofacial development pr... | XR_001751818.2 | 58.9% | (many diffs) | |
| 8 | mouse | 23837 | Cfdp1 | craniofacial development pr... | NM_011801.1 | 84% | 82.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 963
- ORF length:
- 897
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaattcgac tccgaagact tctctacgtc ggaggaggac gaggactacg 121 tgccgtcggg tggagagtat agtgaagatg atgtaaatga attagtgaag gaagatgaag 181 tggatggtga agagcagaca cagaaaaccc aagggaaaaa aagaaaggcc cagagcattc 241 cagccaggaa gagaagacaa ggtggcctct cattagaaga agaggaagag gaggatgcca 301 attcagaatc tgagggaagc agtagtgagg aggaagatga cgctgcagag caggaaaaag 361 gcattggatc agaggatgcc aggaaaaaga aggaggacga actctgggcc agcttcctca 421 atgatgtggg accaaaatca aaagtgcccc caagtacaca agttaagaaa ggagaggaga 481 ctgaagagac aagttcaagt aaattgttgg taaaagcaga agagctagag aaacctaaag 541 aaacagaaaa agttaaaaTC ACCAAGGTGT TTGATTTTGC TGGTGAAGAA GTAAGGGTAA 601 CTAAGGAAGT GGATGCTACA TCTAAAGAGG CCAAATCCTT CTTCAAGCAG AATGAGAAAG 661 AAAAACCACA GGCTAATGTT CCTTCAGCTC TGCCATCACT CCCTGCCGGG TCAGGGTTAA 721 AAAGATCAAG TGGCATGAGC AGCCTTTTGG GGAAAATTGG TGCCAAGAAG CAGAAAATGA 781 GCACCCTTGA GAAGTCCAAA CTGGACTGGG AGAGCTTCAA GGAGGAAGAG GGGATTGGTG 841 AAGAACTGGC CATCCATAAT CGAGGGAAAG AGGGGTACAT TGAACGGAAA GCCTTCCTCG 901 ACCGAGTGGA TCACAGGCAG TTTGAAATTG AGCGAGATCT CAGGCTGAGA AAAATGAAAC 961 CTTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1021 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1081 GGCTTTATAT ATCTTGTGGA AAGGACGACG TGACAAATGC GGATTCCTGC CGACGCGTTA 1141 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt