Construct: ORF TRCN0000479923

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004542.2_s317c1
Derived from:
ccsbBroadEn_02499
DNA Barcode:
ACCACACCGTTTGGGACACATCTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CTCF (10664)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479923
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10664 CTCF CCCTC-binding factor NM_006565.4 100% 100%
2 human 10664 CTCF CCCTC-binding factor XM_017022868.1 100% 100%
3 human 10664 CTCF CCCTC-binding factor NM_001363916.1 99.7% 99.7% 1999_2000insCAACAG
4 human 10664 CTCF CCCTC-binding factor XM_005255775.4 99.7% 99.7% 1999_2000insCAACAG
5 human 10664 CTCF CCCTC-binding factor NM_001191022.2 54.8% 54.8% 0_1ins984
6 mouse 13018 Ctcf CCCTC-binding factor XM_017312557.1 91.6% 98% (many diffs)
7 mouse 13018 Ctcf CCCTC-binding factor NM_181322.3 91.5% 97.9% (many diffs)
8 mouse 13018 Ctcf CCCTC-binding factor XM_006530648.2 91.5% 97.9% (many diffs)
9 mouse 13018 Ctcf CCCTC-binding factor XM_006530645.3 90.5% 96.9% (many diffs)
10 mouse 13018 Ctcf CCCTC-binding factor XM_006530644.2 90.4% 96.7% (many diffs)
11 mouse 13018 Ctcf CCCTC-binding factor XM_006530647.2 90.2% 96.6% (many diffs)
12 mouse 13018 Ctcf CCCTC-binding factor XM_006530646.3 90.1% 96.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2247
ORF length:
2181
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga aggtgatgca gtcgaagcca ttgtggagga gtccgaaact tttattaaag
121gaaaggagag aaagacttac cagagacgcc gggaaggggg ccaggaagaa gatgcctgcc
181acttacccca gaaccagacg gatgggggtg aggtggtcca ggatgtcaac agcagtgtac
241agatggtgat gatggaacag ctggacccca cccttcttca gatgaagact gaagtaatgg
301agggcacagt ggctccagaa gcagaggctg ctgtggacga tacccagatt ataactttac
361aggttgtaaa tatggaggaa cagcccataa acataggaga acttcagctt gttcaagtac
421ctgttcctgt gactgtacct gttgctacca cttcagtaga agaacttcag ggggcttatg
481aaaatgaagt gtctaaagag ggccttgcgg aaagtgaacc catgatatgc cacaccctac
541ctttgcctga agggtttcag gtggttaaag tgggggccaa tggagaggtg gagacactag
601aacaagggga acttccaccc caggaagatc ctagttggca aaaagaccca gactatcagc
661caccagccaa aaaaacaaag aaaaccaaaa agagcaaact gcgttataca gaggagggca
721aagatgtaga tgtgtctgtc tacgattttg aggaagaaca gcaggagggt ctgctatcag
781aggttaatgc agagaaagtg gttggtaata tgaagcctcc aaagccaaca aaaattaaaa
841agaaaggtgt aaagaagaca ttccagtgtg agctttgcag ttacacgtgt ccacggcgtt
901caaatttgga tcgtcacatg aaaagccaca ctgatgagag accacacaag tgccatctct
961gtggcagggc attcagaaca gtcaccctcc tgaggaatca ccttaacaca cacacaggta
1021ctcgtcctca caagtgccca gactgcgaca tggcctttgt gaccagtgga gaattggttc
1081ggcatcgtcg ttacaaacac acccacgaga agccattcaa gtgttccatg tgcgattacg
1141ccagtgtaga agtcagcaaa ttaaaacgtc acattcgctc tcatactgga gagcgtccgt
1201ttcagtgcag tttgtgcagt tatgccagca gggacacata caagctgaaa aggcacatga
1261gaacccattc aggggaaaag ccttatgaat gttatatttg tcatgctcgg tttacccaaa
1321gtggtaccat gaagatgcac attttacaga agcacacaga aaatgtggcc aaatttcact
1381gtccccactg tgacacagtc atagcccgaa aaagtgattt gggtgtccac ttgcgaaagc
1441agcattccta tattgagcaa ggcaagaaat gccgttactg tgatgctgtg tttcatgagc
1501gctatgccct catccagcat cagaagtcac acaagaatga gaagcgcttt aagtgtgacc
1561agtgtgatta cgcttgtaga caggagaggc acatgatcat gcacaagcgc acccacaccg
1621gggagaagcc ttacgcctgc agccactgcg ataagacctt ccgccagaag cagcttctcg
1681acatgcactt caagcgctat cacgacccca acttcgtccc tgcggctttt gtctgttcta
1741agtgtgggaa aacatttaca cgtcggaata ccatggcaag acatgctgat aattgtgctg
1801gcccagatgg cgtagagggg gaaaatggag gagaaacgaa gaagagtaaa cgtggaagaa
1861aaagaaagat gcgctctaag aaagaagatt cctctgacag tgaaaatgct gaaccagatc
1921tggacgacaa tgaggatgag gaggagcctg ccgtagaaat tgaacctgag ccagagccTC
1981AGCCTGTGAC CCCAGCCCCA CCACCCGCCA AGAAGCGGAG AGGACGACCC CCTGGCAGAA
2041CCAACCAGCC CAAACAGAAC CAGCCAACAG CTATCATTCA GGTTGAAGAC CAGAATACAG
2101GTGCAATTGA GAACATTATA GTTGAAGTAA AAAAAGAGCC AGATGCTGAG CCCGCAGAGG
2161GAGAGGAAGA GGAGGCCCAG CCAGCTGCCA CAGATGCCCC CAACGGAGAC CTCACGCCCG
2221AGATGATCCT CAGCATGATG GACCGGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG
2281GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
2341AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACCACACC
2401GTTTGGGACA CATCTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
2461aagatt

Download FASTA (ORF) (Full)