Construct: ORF TRCN0000479930
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015215.1_s317c1
- Derived from:
- ccsbBroadEn_00784
- DNA Barcode:
- CCTCCTTCCTCATTCCATATGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HRAS (3265)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479930
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3265 | HRAS | HRas proto-oncogene, GTPase | NM_176795.4 | 100% | 100% | |
| 2 | human | 3265 | HRAS | HRas proto-oncogene, GTPase | NM_001130442.2 | 85.2% | 80.4% | (many diffs) |
| 3 | human | 3265 | HRAS | HRas proto-oncogene, GTPase | NM_005343.4 | 85.2% | 80.4% | (many diffs) |
| 4 | human | 3265 | HRAS | HRas proto-oncogene, GTPase | NM_001318054.2 | 29.4% | 6.3% | 0_1ins319;192_330del |
| 5 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | NM_001130444.1 | 78.9% | 87.3% | (many diffs) |
| 6 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | NM_001130443.1 | 76.2% | 80.4% | (many diffs) |
| 7 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | NM_008284.2 | 76.2% | 80.4% | (many diffs) |
| 8 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | XM_006536159.2 | 76.2% | 80.4% | (many diffs) |
| 9 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | XR_389950.2 | 40% | (many diffs) | |
| 10 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | XR_389951.2 | 38.9% | (many diffs) | |
| 11 | mouse | 15461 | Hras | Harvey rat sarcoma virus on... | XR_389952.2 | 35.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 576
- ORF length:
- 510
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ggaatataag ctggtggtgg tgggcgccgg cggtgtgggc aagagtgcgc 121 tgaccatcca gctgatccag aaccattttg tggacgaata cgaccccact atagaggatt 181 cctaccggaa gcaggtggtc attgatgggg agacgtgcct gttggacatc ctggataccg 241 ccggCCAGGA GGAGTACAGC GCCATGCGGG ACCAGTACAT GCGCACCGGG GAGGGCTTCC 301 TGTGTGTGTT TGCCATCAAC AACACCAAGT CTTTTGAGGA CATCCACCAG TACAGGGAGC 361 AGATCAAACG GGTGAAGGAC TCGGATGACG TGCCCATGGT GCTGGTGGGG AACAAGTGTG 421 ACCTGGCTGC ACGCACTGTG GAATCTCGGC AGGCTCAGGA CCTCGCCCGA AGCTACGGCA 481 TCCCCTACAT CGAGACCTCG GCCAAGACCC GGCAGGGCAG CCGCTCTGGC TCTAGCTCCA 541 GCTCCGGGAC CCTCTGGGAC CCCCCGGGAC CCATGTGCCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 ACCTCCTTCC TCATTCCATA TGCCAACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt