Construct: ORF TRCN0000479934
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013518.2_s317c1
- Derived from:
- ccsbBroadEn_08052
- DNA Barcode:
- CGCCCACCCGAAACGGTCGTTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CACNG4 (27092)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479934
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27092 | CACNG4 | calcium voltage-gated chann... | NM_014405.4 | 99.8% | 100% | 750C>T |
| 2 | mouse | 54377 | Cacng4 | calcium channel, voltage-de... | NM_019431.2 | 92.2% | 98.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1047
- ORF length:
- 981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gcgatgcgac cgcgggctgc agatgctgct gaccacggcc ggagccttcg 121 ccgccttctc gctcatggcc atcgccatcg gcaccgacta ctggctgtac tccagcgcgc 181 acatctgcaa cggcaccaac ctgaccatgg acgacgggcc cccgccccgc cgcgcccgcg 241 gcgacctcac ccactctggt ctgtggcggg tgtgctgcat cgaagggatc tataaagggc 301 actgcttccg gatcaatcac ttcccagagg acaatgacta cgaccacgac agctcggagt 361 acctcctccg catcgtgcga gcctccagcg tcttccccat cctcagcacc atcctgctcc 421 tgctgggtgg cctgtgcatc ggtgctggca ggatctacag ccgcaagaac aacatcgtcc 481 tcagtgccgg catcctcttc gtggctgcag gcctcagtaa catcatcggt atcatcgtct 541 acatttccag caacacaggt gacccgagtg acaagcggga cgaagacaaa aagaaccatt 601 acaactacgg ctggtctttt tactttGGAG CTCTGTCTTT CATTGTGGCT GAGACCGTGG 661 GCGTCCTGGC TGTAAACATT TACATTGAGA AAAATAAAGA GTTGAGGTTT AAGACCAAAC 721 GGGAATTCCT TAAGGCGTCT TCCTCTTCTC CTTATGCCAG GATGCCGAGC TACAGGTACC 781 GGCGACGGCG CTCGAGGTCC AGCTCAAGGT CCACTGAGGC CTCGCCCTCC AGGGACGTGT 841 CGCCCATGGG CCTGAAGATC ACAGGGGCCA TCCCCATGGG GGAGCTGTCC ATGTACACGC 901 TGTCCAGGGA GCCCCTCAAG GTGACCACCG CAGCCAGCTA CAGCCCCGAC CAGGAGGCCA 961 GCTTCCTGCA GGTGCATGAC TTTTTCCAGC AGGACCTGAA GGAAGGTTTC CACGTCAGCA 1021 TGCTGAACCG ACGGACGACC CCTGTGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1081 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1141 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGCCCACC 1201 CGAAACGGTC GTTTTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1261 aagatt