Construct: ORF TRCN0000479934

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013518.2_s317c1
Derived from:
ccsbBroadEn_08052
DNA Barcode:
CGCCCACCCGAAACGGTCGTTTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CACNG4 (27092)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479934
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27092 CACNG4 calcium voltage-gated chann... NM_014405.4 99.8% 100% 750C>T
2 mouse 54377 Cacng4 calcium channel, voltage-de... NM_019431.2 92.2% 98.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1047
ORF length:
981
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt gcgatgcgac cgcgggctgc agatgctgct gaccacggcc ggagccttcg
121ccgccttctc gctcatggcc atcgccatcg gcaccgacta ctggctgtac tccagcgcgc
181acatctgcaa cggcaccaac ctgaccatgg acgacgggcc cccgccccgc cgcgcccgcg
241gcgacctcac ccactctggt ctgtggcggg tgtgctgcat cgaagggatc tataaagggc
301actgcttccg gatcaatcac ttcccagagg acaatgacta cgaccacgac agctcggagt
361acctcctccg catcgtgcga gcctccagcg tcttccccat cctcagcacc atcctgctcc
421tgctgggtgg cctgtgcatc ggtgctggca ggatctacag ccgcaagaac aacatcgtcc
481tcagtgccgg catcctcttc gtggctgcag gcctcagtaa catcatcggt atcatcgtct
541acatttccag caacacaggt gacccgagtg acaagcggga cgaagacaaa aagaaccatt
601acaactacgg ctggtctttt tactttGGAG CTCTGTCTTT CATTGTGGCT GAGACCGTGG
661GCGTCCTGGC TGTAAACATT TACATTGAGA AAAATAAAGA GTTGAGGTTT AAGACCAAAC
721GGGAATTCCT TAAGGCGTCT TCCTCTTCTC CTTATGCCAG GATGCCGAGC TACAGGTACC
781GGCGACGGCG CTCGAGGTCC AGCTCAAGGT CCACTGAGGC CTCGCCCTCC AGGGACGTGT
841CGCCCATGGG CCTGAAGATC ACAGGGGCCA TCCCCATGGG GGAGCTGTCC ATGTACACGC
901TGTCCAGGGA GCCCCTCAAG GTGACCACCG CAGCCAGCTA CAGCCCCGAC CAGGAGGCCA
961GCTTCCTGCA GGTGCATGAC TTTTTCCAGC AGGACCTGAA GGAAGGTTTC CACGTCAGCA
1021TGCTGAACCG ACGGACGACC CCTGTGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG
1081GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1141AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGCCCACC
1201CGAAACGGTC GTTTTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1261aagatt

Download FASTA (ORF) (Full)