Construct: ORF TRCN0000479948
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016186.1_s317c1
- Derived from:
- ccsbBroadEn_01006
- DNA Barcode:
- TATGACATTGGACCAAAACCCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MGAT3 (4248)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479948
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4248 | MGAT3 | beta-1,4-mannosyl-glycoprot... | NM_001098270.1 | 100% | 100% | |
| 2 | human | 4248 | MGAT3 | beta-1,4-mannosyl-glycoprot... | NM_002409.5 | 100% | 100% | |
| 3 | mouse | 17309 | Mgat3 | mannoside acetylglucosaminy... | NM_010795.4 | 85.9% | 92.3% | (many diffs) |
| 4 | mouse | 17309 | Mgat3 | mannoside acetylglucosaminy... | XM_006520554.3 | 85.9% | 92.3% | (many diffs) |
| 5 | mouse | 17309 | Mgat3 | mannoside acetylglucosaminy... | XM_006520555.1 | 85.9% | 92.3% | (many diffs) |
| 6 | mouse | 17309 | Mgat3 | mannoside acetylglucosaminy... | XM_006520556.3 | 85.9% | 92.3% | (many diffs) |
| 7 | mouse | 17309 | Mgat3 | mannoside acetylglucosaminy... | XM_006520557.3 | 85.9% | 92.3% | (many diffs) |
| 8 | mouse | 17309 | Mgat3 | mannoside acetylglucosaminy... | XM_011245485.2 | 85.9% | 92.3% | (many diffs) |
| 9 | mouse | 17309 | Mgat3 | mannoside acetylglucosaminy... | XM_006520553.1 | 84.6% | 91% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1668
- ORF length:
- 1599
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagatgaga cgctacaagc tctttctcat gttctgtatg gccggcctgt 121 gcctcatctc cttcctgcac ttcttcaaga ccctgtccta tgtcaccttc ccccgagaac 181 tggcctccct cagccctaac ctggtgtcca gctttttctg gaacaatgcc ccggtcacgc 241 cccaggccag ccccgagcca ggaggccctg acctgctgcg taccccactc tactcccact 301 cgcccctgct gcagccgctg ccgcccagca aggcggccga ggagctccac cgggtggact 361 tggtgctgcc cgaggacacc accgagtatt tcgtgcgcac caaggccggc ggcgtctgct 421 tcaaacccgg caccaagatg ctggagaggc cgcccccggg acggccggag gagaagcctg 481 agggggccaa cggctcctcg gcccggcggc caccccggta cctcctgagc gcccgggagc 541 gcacgggggg ccgaggcgcc cggcgcaagt gggtggagtg cgtgtgcctg cccggctggc 601 acggacccag ctgcggcgtg cccactgtgg tgcagtactc caacctgccc accaaggagc 661 ggctggtgcc cagggaggtg ccgcgccgcg tcatcaacgc catcaacgtc aaccacgagt 721 tcgacctgct ggacgtgcgc ttccacgagc tgggcgacgt ggtggacgcc tttgtggtgt 781 gcgagtccaa cttcacggct tatggggagc cgcggccgct caagttccgg gagatgctga 841 ccaatggcac cttcgagtac atccgccaca aggtgctcta tgtcttcctg gaccacttcc 901 cgcccggcgg ccggcaggac ggctggatcg ccgacgacta cctgcgcacc ttcctcaccc 961 aggacggcgt ctcgcggctg cgcaacctgc ggcccgacga cgtcttcatc attgacgatg 1021 cggacgagat cccggcccgt gacggcgtcc ttttcctcaa gctctacgat ggctggaccg 1081 agcccttcgc cttccacatg cgcaagtcgc tctacggctt cttctggaag cagccgggca 1141 ccctggaggt ggtgtcaggc tgcacggtgg acatgctgca ggcagtgtat gggctggacg 1201 gcatccgcct gcgccgccgc cagtactaca ccatgcccaa cttcagacag tatgagaacc 1261 gcaccggcca catcctggtg cagtggtcgc tgggcagccc CCTGCACTTC GCCGGCTGGC 1321 ACTGCTCCTG GTGCTTCACG CCCGAGGGCA TCTACTTCAA GCTCGTGTCC GCCCAGAATG 1381 GCGACTTCCC ACGCTGGGGT GACTACGAGG ACAAGCGGGA CCTGAACTAC ATCCGCGGCC 1441 TGATCCGCAC CGGGGGCTGG TTCGACGGCA CGCAGCAGGA GTACCCGCCT GCAGACCCCA 1501 GCGAGCACAT GTATGCGCCC AAGTACCTGC TGAAGAACTA CGACCGGTTC CACTACCTGC 1561 TGGACAACCC CTACCAGGAG CCCAGGAGCA CGGCGGCGGG CGGGTGGCGC CACAGGGGTC 1621 CCGAGGGAAG GCCGCCCGCC CGGGGCAAAC TGGACGAGGC GGAAGTCTTG CCAACTTTCT 1681 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1741 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1801 GTGGAAAGGA CGATATGACA TTGGACCAAA ACCCTACACG CGTTAAGTCg acaatcaacc 1861 tctggattac aaaatttgtg aaagatt