Construct: ORF TRCN0000479972
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008113.1_s317c1
- Derived from:
- ccsbBroadEn_05110
- DNA Barcode:
- TCTGCCTGTCGATCGAATCTACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFAP161 (161502)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479972
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 161502 | CFAP161 | cilia and flagella associat... | NM_173528.4 | 100% | 100% | |
| 2 | human | 161502 | CFAP161 | cilia and flagella associat... | XM_006720408.2 | 91.6% | 91.6% | 0_1ins75 |
| 3 | human | 161502 | CFAP161 | cilia and flagella associat... | XM_017021963.1 | 91.6% | 91.6% | 0_1ins75 |
| 4 | human | 161502 | CFAP161 | cilia and flagella associat... | NM_001353365.2 | 71.8% | 70.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 972
- ORF length:
- 903
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgcagaac gtgtatggtc cgggagtccg gataggcaac tggaatgagg 121 atgtctacct ggaggaggag ctcatgaaag acttcttaga gaagagagac aaggggaaac 181 ttctcataca gagaagtaga agactaaaac agaatctctt gagaccgatg caactttccg 241 taactgaaga tggctatatt cattacggtg acaaagtgat gcttgtgaat cctgatgatc 301 ctgacacaga agctgatgtg tttctgcgtg gggacctgag cctgtgtatg actccagatg 361 aaattcagtc ccatctgaaa gacgaattag aggtaccctg tggcctgagc gcagttcaag 421 ccaagacccc aattggcaga aacactttta tcattttgag tgtacacaga gatgccactg 481 gtcaagtcct tagatatggg caggactttt gcctggggat aacaggagga tttgacaaca 541 aaatgttgta tttgtcaagt gaccacagga ccctcctgaa atcgtctaag aggtcttggc 601 tccaggaagt gtacctaaCA GATGAGGTCT CCCATGTGAA CTGCTGGCAG GCTGCCTTCC 661 CTGACCCCCA GTTACGCCTG GAATATGAAG GCTTCCCCGT CCCGGCAAAT GCTAAAATTC 721 TCATCAATCA CTGTCACACA AATCGGGGAC TGGCAGCCCA CCGGCATCTT TTCTTAAGCA 781 CCTATTTTGG AAAGGAGGCT GAGGTTGTAG CTCACACATA CCTGGATTCA CATAGAGTTG 841 AGAAACCAAG GAACCACTGG ATGTTGGTTA CTGGGAATCC CAGGGATGCC TCGTCCTCCA 901 TGTTGGATCT GCCCAAACCA CCCACAGAGG ACACTCGAGC CATGGAGCAG GCCATGGGCC 961 TTGACACGCA GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1021 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1081 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCT GCCTGTCGAT CGAATCTACT 1141 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t