Construct: ORF TRCN0000479975

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006355.1_s317c1
Derived from:
ccsbBroadEn_08249
DNA Barcode:
GATACCGCCTGAGCAGCGAGACTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NIN (51199)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479975
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51199 NIN ninein NM_016350.4 99.9% 100% 2835G>C;3498G>A
2 human 51199 NIN ninein XM_011536822.2 94.4% 92.9% (many diffs)
3 human 51199 NIN ninein XR_001750350.2 84.7% (many diffs)
4 human 51199 NIN ninein XR_001750348.2 83.4% (many diffs)
5 human 51199 NIN ninein NM_182946.1 65.8% 65.8% 2398_4536del;4974G>C;5637G>A
6 human 51199 NIN ninein XM_011536823.2 64.6% 63.8% (many diffs)
7 human 51199 NIN ninein NM_020921.3 64.2% 63.3% (many diffs)
8 human 51199 NIN ninein XM_024449622.1 63.3% 62.4% (many diffs)
9 human 51199 NIN ninein NM_182944.2 63.1% 63% (many diffs)
10 human 51199 NIN ninein XM_011536819.3 62.2% 62.1% (many diffs)
11 human 51199 NIN ninein XR_001750345.2 57.8% (many diffs)
12 human 51199 NIN ninein XR_001750349.2 50.2% (many diffs)
13 human 51199 NIN ninein XR_001750347.2 49.8% (many diffs)
14 human 51199 NIN ninein XR_001750346.2 39.8% (many diffs)
15 human 51199 NIN ninein XR_001750344.2 39.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4200
ORF length:
4131
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggatgaggtg gagcaggacc agcatgaggc ccgactcaag gagctgtttg
121acagttttga cacgacgggc acagggtccc tggggcagga ggaactcacc gacctttgcc
181acatgttgag cttggaggag gtggccccag tgctgcagca gacattactt caggacaacc
241tcttgggcag ggtacatttt gaccaattta aagaagcatt aatactcatc ttgtccagaa
301ctctgtcaaa tgaagaacac tttcaagaac cagactgctc actagaagct cagcccaaat
361atgttagagg tgggaagcgt tacggacgaa ggtccttgcc cgagttccaa gagtccgtgg
421aggagtttcc tgaagtgacg gtgattgagc cactggatga agaagcgcgg ccttcacaca
481tcccagccgg tgactgcagt gagcactgga agacgcaacg cagtgaggag tatgaagcgg
541aaggccagtt aaggttttgg aacccagatg acttgaatgc ttcacagagt ggatcttccc
601ctccccaaga ctggatagaa gagaaactgc aagaagtttg tgaagatttg gggatcaccc
661gtgatggtca cctgaaccgg aagaagctgg tctccatctg tgagcagtat ggtttacaga
721atgtggatgg agagatgctc gaggaagtat tccataatct tgatcctgac ggtacaatga
781gtgtagaaga ttttttctat ggtttgttta aaaatggaaa atctcttaca ccatcagcat
841ctactccata tagacaacta aaaaggcacc tttccatgca gtctttcgat gagagtggac
901gacgtaccac aacctcatca gcaatgacaa gtaccattgg ctttcgggtc ttctcctgcc
961tggatgatgg gatgggccat gcatctgtgg agagaatact ggacacctgg caggaagagg
1021gcattgagaa cagccaggag atcctgaagg ccttggattt cagcctcgat ggaaacatca
1081atttgacaga attaacactg gcccttgaaa atgaactttt ggttaccaag aacagcattc
1141accaggcggc tctggccagc tttaaggctg aaatccggca tttgttggaa cgagttgatc
1201aggtggtcag agaaaaagag aagctacggt cagatctgga caaggccgag aagctcaagt
1261ctttaatggc ctcggaggtg gatgatcacc atgcggccat agagcggcgg aatgagtaca
1321acctcaggaa actggatgaa gagtacaagg agcgaatagc agccttaaaa aatgaactcc
1381gaaaagagag agagcagatc ctgcagcagg caggcaagca gcgtttagaa cttgaacagg
1441aaattgaaaa ggcaaaaaca gaagagaact atatccggga ccgccttgcc ctctctttaa
1501aggaaaacag tcgtctggaa aatgagcttc tagaaaatgc agagaagttg gcagaatatg
1561agaatctgac aaacaaactt cagagaaatt tggaaaatgt gttagcagaa aagtttggtg
1621acctcgatcc tagcagtgct gagttcttcc tgcaagaaga gagactgaca cagatgagaa
1681atgaatatga gcggcagtgc agggtactac aagaccaagt agatgaactc cagtctgagc
1741tggaagaata tcgtgcacaa ggcagagtgc tcaggcttcc gttgaagaac tcaccgtcag
1801aagaagttga ggctaacagc ggtggcattg agcccgaaca cgggctcggt tctgaagaat
1861gcaatccatt gaatatgagc attgaggcag agctggtcat tgaacagatg aaagaacaac
1921atcacaggga catatgttgc ctcagactgg agctcgaaga taaagtgcgc cattatgaaa
1981agcagctgga cgaaaccgtg gtcagctgca agaaggcaca ggagaacatg aagcaaaggc
2041atgagaacga aacgcacacc ttagaaaaac aaataagtga ccttaaaaat gaaattgctg
2101aacttcaggg gcaagcagca gtgctcaagg aggcacatca tgaggccact tgcaggcatg
2161aggaggagaa aaaacaactg caagtgaagc ttgaggagga aaagactcac ctgcaggaga
2221agctgaggct gcaacatgag atggagctca aggctagact gacacaggct caagcaagct
2281ttgagcggga gagggaaggc cttcagagta gcgcctggac agaagagaag gtgagaggct
2341tgactcagga actagagcag tttcaccagg agcagctgac aagcctggtg gagaaacaca
2401ctcttgagaa agaggagtta agaaaagagc tcttggaaaa gcaccaaagg gagcttcagg
2461agggaagata tgaatctgaa aagcttcaac aggaaaattc tattttgaga aatgaaatta
2521ctactttaaa tgaagaagat agcatttcta acctgaaatt agggacatta aatggatctc
2581aggaagaaat gtggcaaaaa acggaaactg taaaacaaga aaatgctgca gttcagaaga
2641tggttgaaaa tttaaagaaa cagatttcag aattaaaaat caaaaaccaa caattggatt
2701tggaaaatac agaacttagc caaaagaact ctcaaaacca ggaaaaactg caagaactta
2761atcaacgtct aacagaaatg ctatgccaga aggaaaaaga gccaggaaac agtgcattgg
2821aggaacggga acaagagaag tttaatctga aagaagaact ggaacgttgt aaagtgcagt
2881cctccacttt agtgtcttct ctcgaggcgg agctctctga agttaaaata cagacccata
2941ttgtgcaaca ggaaaaccac cttctcaaag atgaactgga gaaaatgaaa cagctgcaca
3001gatgtcccga tctctctgac ttccagcaaa aaatctctag tgttctaagc tacaacgaaa
3061aactgctgaa agaaaaggaa gctctgagtg aggaattaaa tagctgtgtc gataagttgg
3121caaaatcaag tcttttagag catagaattg cgacgatgaa gcaggaacag aaatcctggg
3181aacatcagag tgcgagctta aagtcacagc tggtggcttc tcaggaaaag gttcagaatt
3241tagaagacac cgtgcagaat gtaaacctgc aaatgtcccg gatgaaatct gacctacgag
3301tgactcagca ggaaaaggag gctttaaaac aagaagtgat gtctttacat aagcaacttc
3361agaatgctgg tggcaagagc tgggccccag agatagctac tcatccatca gggctccata
3421accagcagaa aaggctgtcc tgggacaagt tggatcatct gatgaatgag gaacagcagc
3481tgctttggca agagaatgag aggctccaga ccatggtaca gaacaccaaa gccgaactca
3541cgcactcccg ggagaaggtc cgtcaattgg aatccaatct tcttcccaag caccaaaaac
3601atctaaaccc atcaggtacc atgaatccca cagagcaaga aaaattgagc ttaaagagag
3661agtgtgatca gtttcagaaa gaacaatctc ctgctaacag gaaggtcagt cagatgaatt
3721cccttgaaca agaattagaa acaattcatt tggaaaatga aggcctgaaa aagaaacaag
3781taaaactgga tgagcagctc atggagatgc agcacctgag gtccactgcg acgcctagcc
3841cgtcccctca tgcttggGAT TTGCAGCTGC TCCAGCAGCA AGCCTGTCCG ATGGTGCCCA
3901GGGAGCAGTT TCTGCAGCTT CAACGCCAGC TGCTGCAGGC AGAAAGGATA AACCAGCACC
3961TGCAGGAGGA ACTTGAAAAC AGGACCTCCG AAACCAACAC ACCACAGGGA AACCAGGAAC
4021AACTGGTAAC TGTCATGGAG GAACGAATGA TAGAAGTTGA ACAGAAACTG AAACTAGTGA
4081AAAGGCTTCT TCAAGAGAAA GTGAATCAGC TCAAAGAACA AGTGAGCCTA CCCGGTCATC
4141TCTGTTCACC CACCTCACAT TCCAGCTTTA ACTCCAGTTT TACATCCCTT TATTGCCATT
4201TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
4261GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
4321TTTATATATC TTGTGGAAAG GACGAGATAC CGCCTGAGCA GCGAGACTTA CGCGTTAAGT
4381Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)