Construct: ORF TRCN0000479975
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006355.1_s317c1
- Derived from:
- ccsbBroadEn_08249
- DNA Barcode:
- GATACCGCCTGAGCAGCGAGACTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NIN (51199)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479975
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51199 | NIN | ninein | NM_016350.4 | 99.9% | 100% | 2835G>C;3498G>A |
| 2 | human | 51199 | NIN | ninein | XM_011536822.2 | 94.4% | 92.9% | (many diffs) |
| 3 | human | 51199 | NIN | ninein | XR_001750350.2 | 84.7% | (many diffs) | |
| 4 | human | 51199 | NIN | ninein | XR_001750348.2 | 83.4% | (many diffs) | |
| 5 | human | 51199 | NIN | ninein | NM_182946.1 | 65.8% | 65.8% | 2398_4536del;4974G>C;5637G>A |
| 6 | human | 51199 | NIN | ninein | XM_011536823.2 | 64.6% | 63.8% | (many diffs) |
| 7 | human | 51199 | NIN | ninein | NM_020921.3 | 64.2% | 63.3% | (many diffs) |
| 8 | human | 51199 | NIN | ninein | XM_024449622.1 | 63.3% | 62.4% | (many diffs) |
| 9 | human | 51199 | NIN | ninein | NM_182944.2 | 63.1% | 63% | (many diffs) |
| 10 | human | 51199 | NIN | ninein | XM_011536819.3 | 62.2% | 62.1% | (many diffs) |
| 11 | human | 51199 | NIN | ninein | XR_001750345.2 | 57.8% | (many diffs) | |
| 12 | human | 51199 | NIN | ninein | XR_001750349.2 | 50.2% | (many diffs) | |
| 13 | human | 51199 | NIN | ninein | XR_001750347.2 | 49.8% | (many diffs) | |
| 14 | human | 51199 | NIN | ninein | XR_001750346.2 | 39.8% | (many diffs) | |
| 15 | human | 51199 | NIN | ninein | XR_001750344.2 | 39.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4200
- ORF length:
- 4131
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatgaggtg gagcaggacc agcatgaggc ccgactcaag gagctgtttg 121 acagttttga cacgacgggc acagggtccc tggggcagga ggaactcacc gacctttgcc 181 acatgttgag cttggaggag gtggccccag tgctgcagca gacattactt caggacaacc 241 tcttgggcag ggtacatttt gaccaattta aagaagcatt aatactcatc ttgtccagaa 301 ctctgtcaaa tgaagaacac tttcaagaac cagactgctc actagaagct cagcccaaat 361 atgttagagg tgggaagcgt tacggacgaa ggtccttgcc cgagttccaa gagtccgtgg 421 aggagtttcc tgaagtgacg gtgattgagc cactggatga agaagcgcgg ccttcacaca 481 tcccagccgg tgactgcagt gagcactgga agacgcaacg cagtgaggag tatgaagcgg 541 aaggccagtt aaggttttgg aacccagatg acttgaatgc ttcacagagt ggatcttccc 601 ctccccaaga ctggatagaa gagaaactgc aagaagtttg tgaagatttg gggatcaccc 661 gtgatggtca cctgaaccgg aagaagctgg tctccatctg tgagcagtat ggtttacaga 721 atgtggatgg agagatgctc gaggaagtat tccataatct tgatcctgac ggtacaatga 781 gtgtagaaga ttttttctat ggtttgttta aaaatggaaa atctcttaca ccatcagcat 841 ctactccata tagacaacta aaaaggcacc tttccatgca gtctttcgat gagagtggac 901 gacgtaccac aacctcatca gcaatgacaa gtaccattgg ctttcgggtc ttctcctgcc 961 tggatgatgg gatgggccat gcatctgtgg agagaatact ggacacctgg caggaagagg 1021 gcattgagaa cagccaggag atcctgaagg ccttggattt cagcctcgat ggaaacatca 1081 atttgacaga attaacactg gcccttgaaa atgaactttt ggttaccaag aacagcattc 1141 accaggcggc tctggccagc tttaaggctg aaatccggca tttgttggaa cgagttgatc 1201 aggtggtcag agaaaaagag aagctacggt cagatctgga caaggccgag aagctcaagt 1261 ctttaatggc ctcggaggtg gatgatcacc atgcggccat agagcggcgg aatgagtaca 1321 acctcaggaa actggatgaa gagtacaagg agcgaatagc agccttaaaa aatgaactcc 1381 gaaaagagag agagcagatc ctgcagcagg caggcaagca gcgtttagaa cttgaacagg 1441 aaattgaaaa ggcaaaaaca gaagagaact atatccggga ccgccttgcc ctctctttaa 1501 aggaaaacag tcgtctggaa aatgagcttc tagaaaatgc agagaagttg gcagaatatg 1561 agaatctgac aaacaaactt cagagaaatt tggaaaatgt gttagcagaa aagtttggtg 1621 acctcgatcc tagcagtgct gagttcttcc tgcaagaaga gagactgaca cagatgagaa 1681 atgaatatga gcggcagtgc agggtactac aagaccaagt agatgaactc cagtctgagc 1741 tggaagaata tcgtgcacaa ggcagagtgc tcaggcttcc gttgaagaac tcaccgtcag 1801 aagaagttga ggctaacagc ggtggcattg agcccgaaca cgggctcggt tctgaagaat 1861 gcaatccatt gaatatgagc attgaggcag agctggtcat tgaacagatg aaagaacaac 1921 atcacaggga catatgttgc ctcagactgg agctcgaaga taaagtgcgc cattatgaaa 1981 agcagctgga cgaaaccgtg gtcagctgca agaaggcaca ggagaacatg aagcaaaggc 2041 atgagaacga aacgcacacc ttagaaaaac aaataagtga ccttaaaaat gaaattgctg 2101 aacttcaggg gcaagcagca gtgctcaagg aggcacatca tgaggccact tgcaggcatg 2161 aggaggagaa aaaacaactg caagtgaagc ttgaggagga aaagactcac ctgcaggaga 2221 agctgaggct gcaacatgag atggagctca aggctagact gacacaggct caagcaagct 2281 ttgagcggga gagggaaggc cttcagagta gcgcctggac agaagagaag gtgagaggct 2341 tgactcagga actagagcag tttcaccagg agcagctgac aagcctggtg gagaaacaca 2401 ctcttgagaa agaggagtta agaaaagagc tcttggaaaa gcaccaaagg gagcttcagg 2461 agggaagata tgaatctgaa aagcttcaac aggaaaattc tattttgaga aatgaaatta 2521 ctactttaaa tgaagaagat agcatttcta acctgaaatt agggacatta aatggatctc 2581 aggaagaaat gtggcaaaaa acggaaactg taaaacaaga aaatgctgca gttcagaaga 2641 tggttgaaaa tttaaagaaa cagatttcag aattaaaaat caaaaaccaa caattggatt 2701 tggaaaatac agaacttagc caaaagaact ctcaaaacca ggaaaaactg caagaactta 2761 atcaacgtct aacagaaatg ctatgccaga aggaaaaaga gccaggaaac agtgcattgg 2821 aggaacggga acaagagaag tttaatctga aagaagaact ggaacgttgt aaagtgcagt 2881 cctccacttt agtgtcttct ctcgaggcgg agctctctga agttaaaata cagacccata 2941 ttgtgcaaca ggaaaaccac cttctcaaag atgaactgga gaaaatgaaa cagctgcaca 3001 gatgtcccga tctctctgac ttccagcaaa aaatctctag tgttctaagc tacaacgaaa 3061 aactgctgaa agaaaaggaa gctctgagtg aggaattaaa tagctgtgtc gataagttgg 3121 caaaatcaag tcttttagag catagaattg cgacgatgaa gcaggaacag aaatcctggg 3181 aacatcagag tgcgagctta aagtcacagc tggtggcttc tcaggaaaag gttcagaatt 3241 tagaagacac cgtgcagaat gtaaacctgc aaatgtcccg gatgaaatct gacctacgag 3301 tgactcagca ggaaaaggag gctttaaaac aagaagtgat gtctttacat aagcaacttc 3361 agaatgctgg tggcaagagc tgggccccag agatagctac tcatccatca gggctccata 3421 accagcagaa aaggctgtcc tgggacaagt tggatcatct gatgaatgag gaacagcagc 3481 tgctttggca agagaatgag aggctccaga ccatggtaca gaacaccaaa gccgaactca 3541 cgcactcccg ggagaaggtc cgtcaattgg aatccaatct tcttcccaag caccaaaaac 3601 atctaaaccc atcaggtacc atgaatccca cagagcaaga aaaattgagc ttaaagagag 3661 agtgtgatca gtttcagaaa gaacaatctc ctgctaacag gaaggtcagt cagatgaatt 3721 cccttgaaca agaattagaa acaattcatt tggaaaatga aggcctgaaa aagaaacaag 3781 taaaactgga tgagcagctc atggagatgc agcacctgag gtccactgcg acgcctagcc 3841 cgtcccctca tgcttggGAT TTGCAGCTGC TCCAGCAGCA AGCCTGTCCG ATGGTGCCCA 3901 GGGAGCAGTT TCTGCAGCTT CAACGCCAGC TGCTGCAGGC AGAAAGGATA AACCAGCACC 3961 TGCAGGAGGA ACTTGAAAAC AGGACCTCCG AAACCAACAC ACCACAGGGA AACCAGGAAC 4021 AACTGGTAAC TGTCATGGAG GAACGAATGA TAGAAGTTGA ACAGAAACTG AAACTAGTGA 4081 AAAGGCTTCT TCAAGAGAAA GTGAATCAGC TCAAAGAACA AGTGAGCCTA CCCGGTCATC 4141 TCTGTTCACC CACCTCACAT TCCAGCTTTA ACTCCAGTTT TACATCCCTT TATTGCCATT 4201 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 4261 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 4321 TTTATATATC TTGTGGAAAG GACGAGATAC CGCCTGAGCA GCGAGACTTA CGCGTTAAGT 4381 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt