Transcript: Human XR_001750345.2

PREDICTED: Homo sapiens ninein (NIN), transcript variant X4, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NIN (51199)
Length:
6946
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001750345.2
NBCI Gene record:
NIN (51199)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001750345.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000425347 ACATCAGAGTGCGAGCTTAAA pLKO_005 5541 3UTR 100% 13.200 18.480 N NIN n/a
2 TRCN0000053746 GCCTGGTTACATGGAACAATT pLKO.1 4516 3UTR 100% 13.200 10.560 N NIN n/a
3 TRCN0000433017 AGAGTACAGAATCAAGTTATA pLKO_005 4558 3UTR 100% 13.200 9.240 N NIN n/a
4 TRCN0000431590 CCTCGATGGAAACATCAATTT pLKO_005 1284 3UTR 100% 13.200 9.240 N NIN n/a
5 TRCN0000421721 GTTAGAGCCAGGGTTAGTAAT pLKO_005 3579 3UTR 100% 13.200 9.240 N NIN n/a
6 TRCN0000053747 GCAGAATGTAAACCTGCAAAT pLKO.1 5613 3UTR 100% 10.800 7.560 N NIN n/a
7 TRCN0000053743 CCCAAATATAAGCTGTTGTAT pLKO.1 4024 3UTR 100% 5.625 3.938 N NIN n/a
8 TRCN0000053744 CCCTTGAACAAGAATTAGAAA pLKO.1 6080 3UTR 100% 5.625 3.938 N NIN n/a
9 TRCN0000053745 CGGCATTTGTTGGAACGAGTT pLKO.1 1396 3UTR 100% 4.050 2.835 N NIN n/a
10 TRCN0000441660 GTTAGAGGTGGGAAGCGTTAT pLKO_005 583 3UTR 100% 10.800 15.120 N Nin n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001750345.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08249 pDONR223 100% 57.8% None (many diffs) n/a
2 ccsbBroad304_08249 pLX_304 0% 57.8% V5 (many diffs) n/a
3 TRCN0000479975 GATACCGCCTGAGCAGCGAGACTT pLX_317 8.4% 57.8% V5 (many diffs) n/a
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