Construct: ORF TRCN0000479985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012183.1_s317c1
- Derived from:
- ccsbBroadEn_14134
- DNA Barcode:
- AGTCTCAATAGTCCTGGATCTAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MRPL4 (51073)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479985
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51073 | MRPL4 | mitochondrial ribosomal pro... | NM_146388.2 | 99.8% | 4.1% | 12_13insG |
| 2 | human | 51073 | MRPL4 | mitochondrial ribosomal pro... | NM_015956.3 | 83.4% | 3.5% | (many diffs) |
| 3 | human | 51073 | MRPL4 | mitochondrial ribosomal pro... | NM_146387.1 | 83.4% | 3.5% | (many diffs) |
| 4 | human | 51073 | MRPL4 | mitochondrial ribosomal pro... | XM_011528045.3 | 72.5% | 8.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 393
- ORF length:
- 327
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gcagttcggt ccgggccggg gcgcgggcct ggcttcggcc taccggcagc 121 cagggcctga gttccctggc ggaagaggca gcgcgtgcga ccgagaaccc ggagcaggtg 181 gcgagcgagg gtctcccgga gcccgtgctg cgcaaagtcg agctcccggt acccactcat 241 cgacgcccag tgcaggcctg ggtcgagtcc ttgcggggct tcgagcagga gcgcgtgggc 301 ctggccgacc tgcaccccga tgttttcgcc accgcgccca ggctggacat actgcaccag 361 gttgctatgt ggcagaagaa cttcaagaga attagctatg ccaagaccaa gacgagagcc 421 gaggtgcggg gcggtggccg gaagccttgg ccgcagaaag gcactgggcg ggcccggcat 481 ggcagcatcc gctcTCCGCT CTGGCGAGGA GGAGGTGTTG CCCATGGCCC CCGGGGCCCC 541 ACAAGTTACT ACTACATGCT GCCCATGAAG GTGCGGGCGC TGGGTCTCAA AGTGGCACTG 601 ACCGTCAAGC TGGCCCAGGA CGACCTGCAC ATCATGGACT CCCTAGAGCT GCCCACCGGA 661 GACCCACAGT ACCTGACAGA GCTGGCGCAC TACCGCCGCT GGGGGGACTC CGTACTCCTC 721 GTGGACTTAA CACACGAGGA GATGCCACAG AGCATCGTGG AGGCCACCTC TAGGCTTAAG 781 ACCTTCAACT TGATCCCGGC TGTTGGTGAG CAAAGAGCCC AGGCCCCTAG AGTGCGCATG 841 TGCAGGCTCC GCTGTTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC 901 CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT 961 ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGTCTCAAT AGTCCTGGAT 1021 CTAATACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt