Construct: ORF TRCN0000479988
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010021.1_s317c1
- Derived from:
- ccsbBroadEn_14488
- DNA Barcode:
- AATCCCAGACTTAAATCATATAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CATSPER4 (378807)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479988
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 378807 | CATSPER4 | cation channel sperm associ... | XM_011541432.3 | 89.5% | 75.2% | (many diffs) |
| 2 | human | 378807 | CATSPER4 | cation channel sperm associ... | NM_198137.1 | 78.8% | 65.8% | (many diffs) |
| 3 | human | 378807 | CATSPER4 | cation channel sperm associ... | XM_011541433.2 | 68.9% | 61.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1041
- ORF length:
- 972
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacctccca tacaggcctc gaggggtggg cggtactcag gaggaccgta 121 tggggtttgg aggggcagta gctgcactga ggggccgccc ctctcccctg cagagtacca 181 ttcacgagtc ctacggtcgg ccagaggagc aagtgctcat caaccgccag gaaatcacga 241 acaaagcgga cgcctgggac atgcaggagt tcatcactca catgtacatc aagcagctgc 301 tccgacaccc cgccttccaa ctgctgctgg ccctgctgct ggtgatcaat gccatcacca 361 tcgctctccg taccaactcc tacctggacc agaaacacta tgagttgttc tctaccatag 421 atgacattgt gctgaccatc cttctttgtg aggttctcct tggctggctc aatggcttct 481 ggattttctg gaaggacggc tggaacatcc tcaacttcat tatcgtcttt atcttgctct 541 tgcggttctt cattaatgaa atcaatattc cctccatcaa ctacactctc agggcgcttc 601 gtctggtgca tgtgtgcatg gcggtggagc ccctcgcccg gatcatccgc gtcatcctgc 661 agtcggtgcc tgacatggcc aatatcatgg tcctcatcct cttcttcatg ctgcatttcc 721 agaacataca ggttgcgctg tacaccctct tcatctgcat cacccaggac ggctgggtgg 781 acatctacag tgacttccag acagagaaga gggaatatgc aatggagatt gggggtgcca 841 tctactttac catcttcatc accatcggtg ccttcattGG CATCAACCTG TTCGTCATCG 901 TGGTGACCAC CAACCTGGAG CAAATGATGA AGGCAGGAGA GCAGGGACAA CAGCAACGAA 961 TAACCTTTAG TGAGACAGGC GCAGAGGAAG AGGAGGAGAA TGACCAGCTG CCACTGGTGC 1021 ATTGTGTGGT CGCCCGCTCG TAGAAATCTG GTCTCCTCCA GGAACCCCTT GCGGGAGGCC 1081 CCCTGTCGAA CCTCTCAGAA AACACGTGTG ACAACTTTTG CTTGGTGCTT GAGGCAATAC 1141 AGGAGAACCT GAGGCAGTAC AAGGAGATCC GAGATGAACT CAACATGTTG CCAACTTTCT 1201 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1261 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1321 GTGGAAAGGA CGAAATCCCA GACTTAAATC ATATAACACG CGTTAAGTCg acaatcaacc 1381 tctggattac aaaatttgtg aaagatt