Construct: ORF TRCN0000479996
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002161.3_s317c1
- Derived from:
- ccsbBroadEn_14922
- DNA Barcode:
- TTTAAGCGACAAGCGATCCGCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K14 (9020)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479996
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9020 | MAP3K14 | mitogen-activated protein k... | NM_003954.5 | 99.9% | 100% | 2706C>G |
| 2 | human | 9020 | MAP3K14 | mitogen-activated protein k... | XM_011525441.2 | 99.9% | 100% | 2706C>G |
| 3 | human | 9020 | MAP3K14 | mitogen-activated protein k... | XR_934591.1 | 60.8% | (many diffs) | |
| 4 | mouse | 53859 | Map3k14 | mitogen-activated protein k... | NM_016896.3 | 83.6% | 84.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2907
- ORF length:
- 2841
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agtgatggaa atggcctgcc caggtgcccc tggctcagca gtggggcagc 121 agaaggaact ccccaaagcc aaggagaaga cgccgccact ggggaagaaa cagagctccg 181 tctacaagct tgaggccgtg gagaagagcc ctgtgttctg cggaaagtgg gagatcctga 241 atgacgtgat taccaagggc acagccaagg aaggctccga ggcagggcca gctgccatct 301 ctatcatcgc ccaggctgag tgtgagaata gccaagagtt cagccccacc ttttcagaac 361 gcattttcat cgctgggtcc aaacagtaca gccagtccga gagtcttgat cagatcccca 421 acaatgtggc ccatgctaca gagggcaaaa tggcccgtgt gtgttggaag ggaaagcgtc 481 gcagcaaagc ccggaagaaa cggaagaaga agagctcaaa gtccctggct catgcaggag 541 tggccttggc caaacccctc cccaggaccc ctgagcagga gagctgcacc atcccagtgc 601 aggaggatga gtctccactc ggcgccccat atgttagaaa caccccgcag ttcaccaagc 661 ctctgaagga accaggcctt gggcaactct gttttaagca gcttggcgag ggcctacggc 721 cggctctgcc tcgatcagaa ctccacaaac tgatcagccc cttgcaatgt ctgaaccacg 781 tgtggaaact gcaccacccc caggacggag gccccctgcc cctgcccacg caccccttcc 841 cctatagcag actgcctcat cccttcccat tccaccctct ccagccctgg aaacctcacc 901 ctctggagtc cttcctgggc aaactggcct gtgtagacag ccagaaaccc ttgcctgacc 961 cacacctgag caaactggcc tgtgtagaca gtccaaagcc cctgcctggc ccacacctgg 1021 agcccagctg cctgtctcgt ggtgcccatg agaagttttc tgtggaggaa tacctagtgc 1081 atgctctgca aggcagcgtg agctcaggcc aggcccacag cctgaccagc ctggccaaga 1141 cctgggcagc aaggggctcc agatcccggg agcccagccc caaaactgag gacaacgagg 1201 gtgtcctgct cactgagaaa ctcaagccag tggattatga gtaccgagaa gaagtccact 1261 gggccacgca ccagctccgc ctgggcagag gctccttcgg agaggtgcac aggatggagg 1321 acaagcagac tggcttccag tgcgctgtca aaaaggtgcg gctggaagta tttcgggcag 1381 aggagctgat ggcatgtgca ggattgacct cacccagaat tgtccctttg tatggagctg 1441 tgagagaagg gccttgggtc aacatcttca tggagctgct ggaaggtggc tccctgggcc 1501 agctggtcaa ggagcagggc tgtctcccag aggaccgggc cctgtactac ctgggccagg 1561 ccctggaggg tctggaatac ctccactcac gaaggattct gcatggggac gtcaaagctg 1621 acaacgtgct cctgtccagc gatgggagcc acgcagccct ctgtgacttt ggccatgctg 1681 tgtgtcttca acctgatggc ctgggaaagt ccttgctcac aggggactac atccctggca 1741 cagagaccca catggctccg gaggtggtgc tgggcaggag ctgcgacgcc aaggtggatg 1801 tctggagcag ctgctgtatg atgctgcaca tgctcaacgg ctgccacccc tggactcagt 1861 tcttccgagg gccgctctgc ctcaagattg ccagcgagcc tccgcctgtg agggagatcc 1921 caccctcctg cgcccctctc acagcccagg ccatccaaga ggggctgagg aaagagccca 1981 tccaccgcgt gtctgcagcg gagctgggag ggaaggtgaa ccgggcacta cagcaagtgg 2041 gaggtctgaa gagcccttgg aggggagaat ataaagaacc aagacatcca ccgccaaatc 2101 aagccaatta ccaccagacc ctccatgccc agccgagaga gctttcgcca agggccccag 2161 ggccccggcc agctgaggag acaacaggca gagcccctaa gctccagcct cctctcccac 2221 cagagccccc agagccaaac aagtctcctc ccttgacttt gagcaaggag gagtctggga 2281 tgtgggaacc cttacctctg tcctccctgg agccagcccc tgccagaaac cccagctcac 2341 cagagcggaa agcaaccgtc ccggagcagg aactgcagca gctggaaata gaattattcc 2401 tcaacagcct gtcccagcca ttttctctgg aggagcagga gcaaattctc tcgtgcctca 2461 gcatcgacag cctctccctg tcggatgaca gtgagaagaa cccatcaaag gcctctcaaa 2521 gctcgcggga cacccTGAGC TCAGGCGTAC ACTCCTGGAG CAGCCAGGCC GAGGCTCGAA 2581 GCTCCAGCTG GAACATGGTG CTGGCCCGGG GGCGGCCCAC CGACACCCCA AGCTATTTCA 2641 ATGGTGTGAA AGTCCAAATA CAGTCTCTTA ATGGTGAACA CCTGCACATC CGGGAGTTCC 2701 ACCGGGTCAA AGTGGGAGAC ATCGCCACTG GCATCAGCAG CCAGATCCCA GCTGCAGCCT 2761 TCAGCTTGGT GACCAAAGAC GGGCAGCCTG TTCGCTACGA CATGGAGGTG CCAGACTCGG 2821 GCATCGACCT GCAGTGCACA CTGGCCCCTG ATGGCAGCTT CGCCTGGAGC TGGAGGGTCA 2881 AGCATGGCCA GCTGGAGAAC AGGCCCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 2941 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3001 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTTAAGCG 3061 ACAAGCGATC CGCGCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3121 aagatt