Construct: ORF TRCN0000480037
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014192.1_s317c1
- Derived from:
- ccsbBroadEn_11371
- DNA Barcode:
- GGCGCATCGACCGGGCATATTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ROCK2 (9475)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480037
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9475 | ROCK2 | Rho associated coiled-coil ... | NM_001321643.2 | 53.9% | 53.9% | 1_1797del |
| 2 | human | 9475 | ROCK2 | Rho associated coiled-coil ... | XM_011510417.2 | 52.7% | 52.7% | 1_1797del;3906_3993delinsC |
| 3 | human | 9475 | ROCK2 | Rho associated coiled-coil ... | XM_017005379.2 | 52.7% | 52.7% | 1_1797del;3906_3993delinsC |
| 4 | human | 9475 | ROCK2 | Rho associated coiled-coil ... | NM_004850.5 | 50.6% | 50.6% | 1_2055del |
| 5 | human | 9475 | ROCK2 | Rho associated coiled-coil ... | XM_017005378.2 | 50% | 50% | 1_2055del;4164_4212delinsC |
| 6 | human | 9475 | ROCK2 | Rho associated coiled-coil ... | XM_005246190.3 | 49.5% | 49.5% | 1_2055del;4164_4251delinsC |
| 7 | mouse | 19878 | Rock2 | Rho-associated coiled-coil ... | XM_006515019.3 | 50% | 53.8% | (many diffs) |
| 8 | mouse | 19878 | Rock2 | Rho-associated coiled-coil ... | XM_006515020.3 | 50% | 53.8% | (many diffs) |
| 9 | mouse | 19878 | Rock2 | Rho-associated coiled-coil ... | NM_009072.2 | 45.7% | 49.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2178
- ORF length:
- 2109
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaatagat atgacatacc aactaaaagt tatacagcag agcctagaac 121 aagaagaagc tgaacataag gccacaaagg cacgactagc agataaaaat aagatctatg 181 agtccatcga agaagccaaa tcagaagcca tgaaagaaat ggagaagaag ctcttggagg 241 aaagaacttt aaaacagaaa gtggagaacc tattgctaga agctgagaaa agatgttctc 301 tattagactg tgacctcaaa cagtcacagc agaaaataaa tgagctcctt aaacagaaag 361 atgtgctaaa tgaggatgtt agaaacctga cattaaaaat agagcaagaa actcagaagc 421 gctgccttac acaaaatgac ctgaagatgc aaacacaaca ggttaacaca ctaaaaatgt 481 cagaaaagca gttaaagcaa gaaaataacc atctcatgga aatgaaaatg aacttggaaa 541 aacaaaatgc tgaacttcga aaagaacgtc aggatgcaga tgggcaaatg aaagagctcc 601 aggatcagct cgaagcagaa cagtatttct caacccttta taaaacacaa gttagggagc 661 ttaaagaaga atgtgaagaa aagaccaaac ttggtaaaga attgcagcag aagaaacagg 721 aattacagga tgaacgggac tctttggctg cccaactgga gatcaccttg accaaagcag 781 attctgagca actggctcgt tcaattgctg aagaacaata ttctgatttg gaaaaagaga 841 agatcatgaa agagctggag atcaaagaga tgatggctag acacaaacag gaacttacgg 901 aaaaagatgc tacaattgct tctcttgagg aaactaatag gacactaact agtgatgttg 961 ccaatcttgc aaatgagaaa gaagaattaa ataacaaatt gaaagatgtt caagagcaac 1021 tgtcaagatt gaaagatgaa gaaataagcg cagcagctat taaagcacag tttgagaagc 1081 agctattaac agaaagaaca ctcaaaactc aagctgtgaa taagttggct gagatcatga 1141 atcgaaaaga acctgtcaag cgtggtaatg acacagatgt gcggagaaaa gagaaggaga 1201 atagaaagct acatatggag cttaaatctg aacgtgagaa attgacccag cagatgatca 1261 agtatcagaa agaactgaat gaaatgcagg cacaaatagc tgaagagagc cagattcgaa 1321 ttgaactgca gatgacattg gacagtaaag acagtgacat tgagcagctg cggtcacaac 1381 tccaagcctt gcatattggt ctggatagtt ccagtatagg cagtggacca ggggatgctg 1441 aggcagatga tgggtttcca gaatcaagat tagaaggatg gctttcattg cctgtacgaa 1501 acaacactaa gaaatttgga tgggttaaaa agtatgtgat tgtaagcagt aagaagattc 1561 ttttctatga cagtgaacaa gataaagaac aatccaatcc ttacatggtt ttagatatag 1621 acaagttatt tcatgtccga ccagttacac agacagatgt gtatagagca gatgctaaag 1681 aaattccaag gatattccag attctgtatg ccaatgaagg agaaagtaag aaggaacaag 1741 aatttccagt ggagccagtt GGAGAAAAAT CTAATTATAT TTGCCACAAG GGACATGAGT 1801 TTATTCCTAC TCTTTATCAT TTCCCAACCA ACTGTGAGGC TTGTATGAAG CCCCTGTGGC 1861 ACATGTTTAA GCCTCCTCCT GCTTTGGAGT GCCGCCGTTG CCATATTAAG TGTCATAAAG 1921 ATCATATGGA CAAAAAGGAG GAGATTATAG CACCTTGCAA AGTATATTAT GATATTTCAA 1981 CGGCAAAGAA TCTGTTATTA CTAGCAAATT CTACAGAAGA GCAGCAGAAG TGGGTTAGTC 2041 GGTTGGTGAA AAAGATACCT AAAAAGCCCC CAGCTCCAGA CCCTTTTGCC CGATCATCTC 2101 CTAGAACTTC AATGAAGATA CAGCAAAACC AGTCTATTAG ACGGCCAAGT CGACAGCTTG 2161 CCCCAAACAA ACCTAGCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2221 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2281 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGCGCAT CGACCGGGCA 2341 TATTCTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt