Construct: ORF TRCN0000480044
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009458.2_s317c1
- Derived from:
- ccsbBroadEn_12107
- DNA Barcode:
- TGAAGTAAATCCTTTTGCTTGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LAX1 (54900)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480044
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54900 | LAX1 | lymphocyte transmembrane ad... | NM_001282878.1 | 100% | 100% | |
| 2 | human | 54900 | LAX1 | lymphocyte transmembrane ad... | NM_001136190.2 | 84.2% | 84.2% | 1_180del |
| 3 | human | 54900 | LAX1 | lymphocyte transmembrane ad... | NM_017773.4 | 80.9% | 80.9% | 1_228del |
| 4 | human | 54900 | LAX1 | lymphocyte transmembrane ad... | XM_006711397.3 | 80.9% | 80.9% | 1_228del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1032
- ORF length:
- 966
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cttgctgact ttgccacaaa ccagacaaag agccaaaaat atttatgaca 121 tcttgccttg gcgacaggaa gacctgggga gacatgagtc gaggagtatg cgcattttca 181 gtactgagag cctcctctcc agaaattctg agagcccgga gcatgtgccc tcccaagcag 241 gcaatgcctt ccaggagcat acagcccaca tccatgccac agagtacgcg gtgggtatct 301 atgacaacgc catggtcccc cagatgtgtg ggaacctcac tccctcggca cactgcatca 361 atgtcagagc ttccagagac tgcgcaagca tttcttcaga ggattcgcat gattatgtca 421 atgtccccac agcagaagag attgctgaga ctctagcttc taccaaaagc ccttccagaa 481 atctctttgt tcttcccagt acccagaagc tggagtttac tgaggaaaga gatgagggct 541 gtggagatgc tggtgactgc accagtttgt attctccagg agctgaggac agtgattcac 601 tcagcaatgg agaaggttct tctcagatct caaatgacta tgtcaacatg acagggttgg 661 atctcagtgC CATCCAGGAA AGGCAGCTCT GGGTGGCTTT TCAGTGCTGC AGAGACTATG 721 AAAATGTTCC AGCAGCAGAT CCCAGTGGAA GCCAGCAGCA GGCTGAGAAA GATGTGCCAT 781 CCTCAAACAT AGGTCATGTC GAGGACAAGA CAGATGATCC CGGGACCCAT GTCCAATGTG 841 TCAAAAGGAC ATTCCTTGCT TCAGGGGATT ATGCAGACTT TCAGCCATTC ACACAGAGTG 901 AGGACAGTCA GATGAAACAT AGAGAAGAGA TGTCAAATGA GGACTCCAGT GACTATGAAA 961 ATGTGCTAAC TGCCAAGTTA GGAGGCAGGG ACTCTGAGCA GGGGCCTGGC ACTCAGCTCC 1021 TTCCTGATGA ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1081 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1141 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGA AGTAAATCCT TTTGCTTGTT 1201 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t