Transcript: Human NM_017773.4

Homo sapiens lymphocyte transmembrane adaptor 1 (LAX1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-06-30
Taxon:
Homo sapiens (human)
Gene:
LAX1 (54900)
Length:
3271
CDS:
384..1580

Additional Resources:

NCBI RefSeq record:
NM_017773.4
NBCI Gene record:
LAX1 (54900)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_017773.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000142221 GAGACATGAGTCGAGGAGTAT pLKO.1 695 CDS 100% 4.950 3.960 N LAX1 n/a
2 TRCN0000121787 CATAGAGAAGAGATGTCAAAT pLKO.1 1464 CDS 100% 13.200 9.240 N LAX1 n/a
3 TRCN0000144155 CCTTCCAGAAATCTCTTTGTT pLKO.1 1017 CDS 100% 5.625 3.938 N LAX1 n/a
4 TRCN0000143875 CTTCAGAGGATTCGCATGATT pLKO.1 940 CDS 100% 5.625 3.938 N LAX1 n/a
5 TRCN0000143577 GAGGACAGTCAGATGAAACAT pLKO.1 1446 CDS 100% 5.625 3.938 N LAX1 n/a
6 TRCN0000143285 GACAGTGATTCACTCAGCAAT pLKO.1 1134 CDS 100% 4.950 3.465 N LAX1 n/a
7 TRCN0000121856 GATTCGCATGATTATGTCAAT pLKO.1 948 CDS 100% 4.950 3.465 N LAX1 n/a
8 TRCN0000142356 GAGAAAGATGTGCCATCCTCA pLKO.1 1311 CDS 100% 2.640 1.848 N LAX1 n/a
9 TRCN0000122711 CCAGGAGCATACAGCCCACAT pLKO.1 797 CDS 100% 1.350 0.945 N LAX1 n/a
10 TRCN0000144854 GAAATCTCTTTGTTCTTCCCA pLKO.1 1024 CDS 100% 0.750 0.525 N LAX1 n/a
11 TRCN0000142188 GCACACTGCATCAATGTCAGA pLKO.1 894 CDS 100% 2.640 1.584 N LAX1 n/a
12 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 3029 3UTR 100% 4.950 2.475 Y ORAI2 n/a
13 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 3026 3UTR 100% 4.950 2.475 Y LOC339059 n/a
14 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 2197 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_017773.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12107 pDONR223 100% 80.9% 80.9% None 1_228del n/a
2 ccsbBroad304_12107 pLX_304 0% 80.9% 80.9% V5 1_228del n/a
3 TRCN0000480044 TGAAGTAAATCCTTTTGCTTGTTT pLX_317 37.8% 80.9% 80.9% V5 1_228del n/a
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