Construct: ORF TRCN0000480049
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003166.1_s317c1
- Derived from:
- ccsbBroadEn_13682
- DNA Barcode:
- CATGTGCCAAAGCACATCCCAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IDNK (414328)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480049
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 414328 | IDNK | IDNK gluconokinase | NM_001256915.1 | 100% | 100% | |
| 2 | human | 414328 | IDNK | IDNK gluconokinase | NM_001351535.2 | 100% | 100% | |
| 3 | human | 414328 | IDNK | IDNK gluconokinase | XM_017014724.1 | 100% | 100% | |
| 4 | human | 414328 | IDNK | IDNK gluconokinase | NM_001001551.4 | 75.4% | 75.4% | 1_138del |
| 5 | human | 414328 | IDNK | IDNK gluconokinase | XM_006717111.4 | 75% | 75% | 1_141del |
| 6 | human | 414328 | IDNK | IDNK gluconokinase | XM_017014719.1 | 66.3% | 67.1% | (many diffs) |
| 7 | human | 414328 | IDNK | IDNK gluconokinase | XM_006717110.3 | 61.3% | 61.3% | 1_267del |
| 8 | human | 414328 | IDNK | IDNK gluconokinase | NR_046421.2 | 46.7% | 1_136del;560_905del | |
| 9 | human | 414328 | IDNK | IDNK gluconokinase | NR_046422.2 | 27.7% | 1_753del;1177_1522del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 492
- ORF length:
- 423
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GGGAAAAGGC ATACCGCTCA ATGACCAGGA CCGGATTCCA TGGCTCTGTA 121 ACTTGCATGA CATTTTACTA AGAGATGTAG CCTCGGGACA GCGTGTGGTT CTAGCCTGTT 181 CAGCCCTGAA GAAAACGTAC AGAGACATAT TAACACAAGG AAAAGATGGT GTAGCTCTGA 241 AGTGTGAGGA GTCGGGAAAG GAAGCAAAGC AGGCTGAGAT GCAGCTCCTG GTGGTCCATC 301 TGAGCGGGTC GTTTGAGGTC ATCTCTGGAC GCTTACTCAA AAGAGAGGGA CATTTTATGC 361 CCCCTGAATT ATTGCAGTCC CAGTTTGAGA CTCTGGAGCC CCCAGCAGCT CCAGAAAACT 421 TTATCCAAAT AAGTGTGGAC AAAAATGTTT CAGAGATAAT TGCTACAATT ATGGAAACCC 481 TAAAAATGAA ATTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 541 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 601 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAT GTGCCAAAGC ACATCCCAGT 661 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t