Transcript: Human XM_006717110.3

PREDICTED: Homo sapiens IDNK gluconokinase (IDNK), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
IDNK (414328)
Length:
1154
CDS:
114..806

Additional Resources:

NCBI RefSeq record:
XM_006717110.3
NBCI Gene record:
IDNK (414328)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146514 CCTCTCCACAGATCCACCGT pXPR_003 GGG 509 50% 2 0.5158 IDNK IDNK 75865
2 BRDN0001147773 TCTAAACAGAGATGTAGCCT pXPR_003 CGG 673 66% 5 0.064 IDNK IDNK 75866
3 BRDN0001148428 TGCTGATGATTATCACCCGG pXPR_003 AGG 571 56% 3 -0.1414 IDNK IDNK 75867
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006717110.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000121723 CCAAACAGAACTAAGCATAAA pLKO.1 828 3UTR 100% 13.200 9.240 N IDNK n/a
2 TRCN0000143276 GATTCCATGGCTCTGTAACTT pLKO.1 416 CDS 100% 5.625 3.938 N IDNK n/a
3 TRCN0000145322 GAATTATTGCAGTCCCAGTTT pLKO.1 678 CDS 100% 4.950 3.465 N IDNK n/a
4 TRCN0000145215 GACAGACTTGTTTAGGTGTAA pLKO.1 934 3UTR 100% 4.950 3.465 N IDNK n/a
5 TRCN0000121608 GCTCTGTAACTTGCATGACAT pLKO.1 425 CDS 100% 4.950 3.465 N IDNK n/a
6 TRCN0000145460 GTCCAAACAGAACTAAGCATA pLKO.1 826 3UTR 100% 4.950 3.465 N IDNK n/a
7 TRCN0000139700 CCATGGCTCTGTAACTTGCAT pLKO.1 420 CDS 100% 3.000 2.100 N IDNK n/a
8 TRCN0000122220 GAGACATATTAACACAAGGAA pLKO.1 514 CDS 100% 3.000 2.100 N IDNK n/a
9 TRCN0000140068 GATGGTGTAGCTCTGAAGTGT pLKO.1 537 CDS 100% 3.000 2.100 N IDNK n/a
10 TRCN0000145077 GCTACAATTATGGAAACCCTA pLKO.1 774 CDS 100% 2.640 1.848 N IDNK n/a
11 TRCN0000143398 CTGAATTATTGCAGTCCCAGT pLKO.1 676 CDS 100% 2.160 1.296 N IDNK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006717110.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488718 CCCTAAGTTCTTCTAGCTCCTGGC pLX_317 54% 78.2% 74.3% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_13682 pDONR223 100% 61.3% 61.3% None 1_267del n/a
3 ccsbBroad304_13682 pLX_304 0% 61.3% 61.3% V5 1_267del n/a
4 TRCN0000480049 CATGTGCCAAAGCACATCCCAGTC pLX_317 98.6% 61.3% 61.3% V5 1_267del n/a
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