Construct: ORF TRCN0000480059

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012899.1_s317c1
Derived from:
ccsbBroadEn_15415
DNA Barcode:
TAATTCTCCACATACATTCGTCCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FDFT1 (2222)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480059
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2222 FDFT1 farnesyl-diphosphate farnes... NM_001287742.1 100% 100%
2 human 2222 FDFT1 farnesyl-diphosphate farnes... NM_001287743.1 100% 100%
3 human 2222 FDFT1 farnesyl-diphosphate farnes... NM_004462.5 99.9% 100% 631T>C
4 human 2222 FDFT1 farnesyl-diphosphate farnes... NM_001287750.1 84.9% 77.7% (many diffs)
5 human 2222 FDFT1 farnesyl-diphosphate farnes... NM_001287744.1 84.6% 84.6% 0_1ins192
6 human 2222 FDFT1 farnesyl-diphosphate farnes... NM_001287745.1 84.6% 84.6% 0_1ins192
7 human 2222 FDFT1 farnesyl-diphosphate farnes... NM_001287747.1 84.6% 84.6% 0_1ins192
8 human 2222 FDFT1 farnesyl-diphosphate farnes... NM_001287748.1 84.6% 84.6% 0_1ins192
9 human 2222 FDFT1 farnesyl-diphosphate farnes... NM_001287749.1 84.6% 84.6% 0_1ins192
10 human 2222 FDFT1 farnesyl-diphosphate farnes... NM_001287751.1 79.6% 79.6% 0_1ins255
11 human 2222 FDFT1 farnesyl-diphosphate farnes... NM_001287756.1 59.9% 55.5% 0_1insATGGAGTTCGTGAA;62_63ins487
12 mouse 14137 Fdft1 farnesyl diphosphate farnes... NM_010191.3 84.9% 88.2% (many diffs)
13 mouse 14137 Fdft1 farnesyl diphosphate farnes... XM_017315844.1 84.9% 88.2% (many diffs)
14 mouse 14137 Fdft1 farnesyl diphosphate farnes... XM_006518547.3 55.4% 57.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1317
ORF length:
1251
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gttcgtgaaa tgccttggcc accccgaaga gttctacaac ctggtgcgct
121tccggatcgg gggcaagcgg aaggtgatgc ccaagatgga ccaggactcg ctcagcagca
181gcctgaaaac ttgctacaag tatctcaatc agaccagtcg cagtttcgca gctgttatcc
241aggcgctgga tggggaaatg cgcaacgcag tgtgcatatt ttatctggtt ctccgagctc
301tggacacact ggaagatgac atgaccatca gtgtggaaaa gaaggtcccg ctgttacaca
361actttcactc tttcctttac caaccagact ggcggttcat ggagagcaag gagaaggatc
421gccaggtgct ggaggacttc ccaacgatct cccttgagtt tagaaatctg gctgagaaat
481accaaacagt gattgccgac atttgccgga gaatgggcat tgggatggca gagtttttgg
541ataagcatgt gacctctgaa caggagtggg acaagtactg ccactatgtt gctgggctgg
601tcggaattgg cctttcccgt cttttctcag cctcagagtt tgaagacccc ttagttggtg
661aagatacaga acgtgccaac tctatgggcc tgtttctgca gaaaacaaac atcatccgtg
721actatctgga agaccagcaa ggaggaagag agttctggcc tcaagaggtt tggagcaggt
781atgttaagaa gttaggggat tttgctaagc cggagaatat tgacttggcc gtgcagtgcc
841tgaatgaact tataaccaat gcactgcacc acatcccaga tgtcatcacc TACCTTTCGA
901GACTCAGAAA CCAGAGTGTG TTTAACTTCT GTGCTATTCC ACAGGTGATG GCCATTGCCA
961CTTTGGCTGC CTGTTATAAT AACCAGCAGG TGTTCAAAGG GGCAGTGAAG ATTCGGAAAG
1021GGCAAGCAGT GACCCTGATG ATGGATGCCA CCAATATGCC AGCTGTCAAA GCCATCATAT
1081ATCAGTATAT GGAAGAGATT TATCATAGAA TCCCCGACTC AGACCCATCT TCTAGCAAAA
1141CAAGGCAGAT CATCTCCACC ATCCGGACGC AGAATCTTCC CAACTGTCAG CTGATTTCCC
1201GAAGCCACTA CTCCCCCATC TACCTGTCGT TTGTCATGCT TTTGGCTGCC CTGAGCTGGC
1261AGTACCTGAC CACTCTCTCC CAGGTAACAG AAGACTATGT TCAGACTGGA GAACACTGCC
1321CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1381TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1441ATATATCTTG TGGAAAGGAC GATAATTCTC CACATACATT CGTCCAACGC GTTAAGTCga
1501caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)

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