Construct: ORF TRCN0000480100
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013553.2_s317c1
- Derived from:
- ccsbBroadEn_09712
- DNA Barcode:
- AATTAACTCCTGAATATTCTTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GIMAP8 (155038)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480100
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 155038 | GIMAP8 | GTPase, IMAP family member 8 | NM_175571.4 | 100% | 100% | |
| 2 | human | 155038 | GIMAP8 | GTPase, IMAP family member 8 | XM_005249950.4 | 100% | 100% | |
| 3 | human | 155038 | GIMAP8 | GTPase, IMAP family member 8 | XM_005249951.4 | 68.5% | 68.5% | 681_682ins627 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2064
- ORF length:
- 1995
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcagagcag agctgccaga tgtccgaact gcggctcctc ctcctgggaa 121 aatgccgctc gggaaaaagt gccacaggaa atgccattct gggcaaacat gtgttcaagt 181 ccaagttcag tgatcagaca gtgatcaaaa tgtgccagag agagagttgg gtcctgagag 241 aaaggaaggt tgtggtaatt gacacccctg accttttctc ctcaatagct tgtgctgaag 301 acaagcaacg caacatccaa cactgcttgg agctctctgc tcccagcctc catgctctgc 361 tcttggtaat tgccatcggc catttcacaa gggaggatga ggaaacagcc aagggcatcc 421 aacaagtgtt tggagctgaa gccaggaggc acatcattat tgtcttcact cggaaggatg 481 atttggggga tgacttgctg caagatttca ttgaaaaaaa caaacctctc aagcagttgg 541 ttcaagacta tgagggccga tactgcattt tcaacaacaa gaccaatagt aaggatgagc 601 agatcaccca ggtgttggag ctccttcgca aggttgagtc tttggtgaat acgaacggag 661 gaccctatca tgtgaacttc aaaactgaag gcagcaggtt tcaagattgt gtgaatgaag 721 ctgcatctca agagggagac aagccacagg gcccaaggga aaggcagctg cagtccacag 781 gacccgagca gaatccgggg acatcagaac tgacagtcct ccttgtgggg aaacgcggtg 841 ctggaaaaag tgcagcagga aacagcattc tggggaggca ggcctttcag accggattta 901 gtgagcagtc agtaacccag agcttcttgt ctgagagcag aagctggaga aaaaagaaag 961 tttcgatcat tgatgctccg gacatctcat ctttaaagaa cattgactca gaagttagaa 1021 aacacatctg tacaggcccc catgccttcc tgctggtgac accactgggc ttttacacta 1081 agaatgatga ggcagtgctg agcaccatcc aaaacaattt tggagaaaaa ttctttgagt 1141 acatgatcat acttcttacc aggaaagaag atttagggga tcaggatcta gatacgttct 1201 taagaaacag caataaagct ctctatggtc tcatccagaa gtgtaaaaac agatatagtg 1261 ccttcaacta ccgggcaaca ggagaagaag agcaaaggca ggcggacgag ctcctggaaa 1321 aaattgagag catggtgcat cagaatggga acaagcattg tgttttcaga gaaaaagaaa 1381 ccctgaacat tgtccttgtg gggagaagcg ggactgggaa gagtgcgacc gggaactcta 1441 tcctggggag cctcgtcttc acctctcggc tccgggccca gccagtcacc aagaccagcc 1501 agagtggcag gaggacatgg gacggacagg aggtggtggt tgtggacact ccttccttca 1561 accagatgct ggatgtcgaa aaggacccat cccggttaga agaggaggtc aagcgctgtt 1621 tgtcctgctg tgaaaaaggg gacacatttt ttgtcctggt gttccagctg ggacgattca 1681 ctgaagagga caaaaCAGCT GTGGCGAAAC TGGAGGCCAT CTTTGGAGCA GACTTTACGA 1741 AATACGCGAT TATGCTGTTC ACCCGGAAGG AAGACCTAGG GGCGGGGAAT TTGGAAGACT 1801 TCATGAAGAA CTCAGATAAC AAAGCCCTTC GGCGCATTTT TAAAAAGTGT GGGCGGCGAG 1861 TTTGTGCTTT TAACAACAAA GAAACAGGCC AGGCCCAGGA AACCCAGGTG AAAGCTCTTT 1921 TAACAAAGGT CAATGATCTG AGAAAAGAAA GTGGGTGGTC CGGGTATCCC CATACACAGG 1981 AGAACGTCAG CAAACTAATT AAAAATGTCC AGGAAATGTC CCAAGCCGAA AAACTCCTTA 2041 AAAATTTAAT AGGTATTTTA CAATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2101 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2161 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA ATTAACTCCT 2221 GAATATTCTT ATTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2281 att