Construct: ORF TRCN0000480103
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006822.1_s317c1
- Derived from:
- ccsbBroadEn_01090
- DNA Barcode:
- GTGAATTTATGTCTCTATTTATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NFE2L2 (4780)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480103
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4780 | NFE2L2 | nuclear factor, erythroid 2... | NM_006164.5 | 100% | 100% | |
| 2 | human | 4780 | NFE2L2 | nuclear factor, erythroid 2... | NM_001145412.3 | 97.3% | 97.3% | 0_1ins48 |
| 3 | human | 4780 | NFE2L2 | nuclear factor, erythroid 2... | NM_001313900.1 | 97.3% | 97.3% | 0_1ins48 |
| 4 | human | 4780 | NFE2L2 | nuclear factor, erythroid 2... | NM_001313901.1 | 97.3% | 97.3% | 0_1ins48 |
| 5 | human | 4780 | NFE2L2 | nuclear factor, erythroid 2... | NM_001145413.3 | 96.1% | 96.1% | 0_1ins48;352_353ins21 |
| 6 | human | 4780 | NFE2L2 | nuclear factor, erythroid 2... | NM_001313902.1 | 95% | 95% | 310_311ins90 |
| 7 | human | 4780 | NFE2L2 | nuclear factor, erythroid 2... | NM_001313903.1 | 87.9% | 87.9% | 93_94ins219 |
| 8 | human | 4780 | NFE2L2 | nuclear factor, erythroid 2... | NM_001313904.1 | 83.4% | 82.9% | 0_1ins229;9_10ins71 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1881
- ORF length:
- 1815
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ggacttggag ctgccgccgc cgggactccc gtcccagcag gacatggatt 121 tgattgacat actttggagg caagatatag atcttggagt aagtcgagaa gtatttgact 181 tcagtcagcg acggaaagag tatgagctgg aaaaacagaa aaaacttgaa aaggaaagac 241 aagaacaact ccaaaaggag caagagaaag cctttttcgc tcagttacaa ctagatgaag 301 agacaggtga atttctccca attcagccag cccagcacat ccagtcagaa accagtggat 361 ctgccaacta ctcccaggtt gcccacattc ccaaatcaga tgctttgtac tttgatgact 421 gcatgcagct tttggcgcag acattcccgt ttgtagatga caatgaggtt tcttcggcta 481 cgtttcagtc acttgttcct gatattcccg gtcacatcga gagcccagtc ttcattgcta 541 ctaatcaggc tcagtcacct gaaacttctg ttgctcaggt agcccctgtt gatttagacg 601 gtatgcaaca ggacattgag caagtttggg aggagctatt atccattcct gagttacagt 661 gtcttaatat tgaaaatgac aagctggttg agactaccat ggttccaagt ccagaagcca 721 aactgacaga agttgacaat tatcattttt actcatctat accctcaatg gaaaaagaag 781 taggtaactg tagtccacat tttcttaatg cttttgagga ttccttcagc agcatcctct 841 ccacagaaga ccccaaccag ttgacagtga actcattaaa ttcagatgcc acagtcaaca 901 cagattttgg tgatgaattt tattctgctt tcatagctga gcccagtatc agcaacagca 961 tgccctcacc tgctacttta agccattcac tctctgaact tctaaatggg cccattgatg 1021 tttctgatct atcactttgc aaagctttca accaaaacca ccctgaaagc acagcagaat 1081 tcaatgattc tgactccggc atttcactaa acacaagtcc cagtgtggca tcaccagaac 1141 actcagtgga atcttccagc tatggagaca cactacttgg cctcagtgat tctgaagtgg 1201 aagagctaga tagtgcccct ggaagtgtca aacagaatgg tcctaaaaca ccagtacatt 1261 cttctgggga tatggtacaa cccttgtcac catctcaggg gcagagcact cacgtgcatg 1321 atgcccaatg tgagaacaca ccagagaaag aattgcctgt aagtcctggt catcggaaaa 1381 ccccattcac aaaagacaaa cattcaagcc gcttggaggc tcatctcaca agagatgaac 1441 ttagggcaaa agctctccat atcccaTTCC CTGTAGAAAA AATCATTAAC CTCCCTGTTG 1501 TTGACTTCAA CGAAATGATG TCCAAAGAGC AGTTCAATGA AGCTCAACTT GCATTAATTC 1561 GGGATATACG TAGGAGGGGT AAGAATAAAG TGGCTGCTCA GAATTGCAGA AAAAGAAAAC 1621 TGGAAAATAT AGTAGAACTA GAGCAAGATT TAGATCATTT GAAAGATGAA AAAGAAAAAT 1681 TGCTCAAAGA AAAAGGAGAA AATGACAAAA GCCTTCACCT ACTGAAAAAA CAACTCAGCA 1741 CCTTATATCT CGAAGTTTTC AGCATGCTAC GTGATGAAGA TGGAAAACCT TATTCTCCTA 1801 GTGAATACTC CCTGCAGCAA ACAAGAGATG GCAATGTTTT CCTTGTTCCC AAAAGTAAGA 1861 AGCCAGATGT TAAGAAAAAC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1921 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1981 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTGA ATTTATGTCT 2041 CTATTTATTG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt