Construct: ORF TRCN0000480118
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016226.1_s317c1
- Derived from:
- ccsbBroadEn_01835
- DNA Barcode:
- TATGTTGGAGTTCGACTACACTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CXCR4 (7852)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480118
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7852 | CXCR4 | C-X-C motif chemokine recep... | NM_003467.3 | 100% | 100% | |
| 2 | human | 7852 | CXCR4 | C-X-C motif chemokine recep... | NM_001008540.2 | 97% | 97.4% | 1_22delinsA;25C>G;27_28insGGGATCAGT |
| 3 | human | 7852 | CXCR4 | C-X-C motif chemokine recep... | NM_001348060.2 | 95.7% | 95.7% | 0_1ins45 |
| 4 | human | 7852 | CXCR4 | C-X-C motif chemokine recep... | NM_001348059.2 | 91.4% | 90.3% | 16_114del |
| 5 | human | 7852 | CXCR4 | C-X-C motif chemokine recep... | NM_001348056.2 | 81.4% | 82% | 1_225del;228_229insGAGGGGATCAGT |
| 6 | mouse | 12767 | Cxcr4 | chemokine (C-X-C motif) rec... | XM_006529113.3 | 85.6% | 89.9% | (many diffs) |
| 7 | mouse | 12767 | Cxcr4 | chemokine (C-X-C motif) rec... | NM_009911.3 | 85.1% | 89.1% | (many diffs) |
| 8 | mouse | 12767 | Cxcr4 | chemokine (C-X-C motif) rec... | XM_006529114.2 | 84.8% | 88.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1122
- ORF length:
- 1056
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggggatcagt atatacactt cagataacta caccgaggaa atgggctcag 121 gggactatga ctccatgaag gaaccctgtt tccgtgaaga aaatgctaat ttcaataaaa 181 tcttcctgcc caccatctac tccatcatct tcttaactgg cattgtgggc aatggattgg 241 tcatcctggt catgggttac cagaagaaac tgagaagcat gacggacaag tacaggctgc 301 acctgtcagt ggccgacctc ctctttgtca tcacgcttcc cttctgggca gttgatgccg 361 tggcaaactg gtactttggg aacttcctat gcaaggcagt ccatgtcatc tacacagtca 421 acctctacag cagtgtcctc atcctggcct tcatcagtct ggaccgctac ctggccatcg 481 tccacgccac caacagtcag aggccaagga agctgttggc tgaaaaggtg gtctatgttg 541 gcgtctggat ccctgccctc ctgctgacta ttcccgactt catctttgcc aacgtcagtg 601 aggcagatga cagatatatc tgtgaccgct tctaccccaa tgacttgtgg gtggttgtgt 661 tccagtttca gcacatcatg gttggcctta tcctgcctgg tattgtcatc ctgtcctgct 721 attgcattat catctccaag ctgtcacact ccaagggcca ccagaagcgc aaggccctca 781 agaccacagt catccTCATC CTGGCTTTCT TCGCCTGTTG GCTGCCTTAC TACATTGGGA 841 TCAGCATCGA CTCCTTCATC CTCCTGGAAA TCATCAAGCA AGGGTGTGAG TTTGAGAACA 901 CTGTGCACAA GTGGATTTCC ATCACCGAGG CCCTAGCTTT CTTCCACTGT TGTCTGAACC 961 CCATCCTCTA TGCTTTCCTT GGAGCCAAAT TTAAAACCTC TGCCCAGCAC GCACTCACCT 1021 CTGTGAGCAG AGGGTCCAGC CTCAAGATCC TCTCCAAAGG AAAGCGAGGT GGACATTCAT 1081 CTGTTTCCAC TGAGTCTGAG TCTTCAAGTT TTCACTCCAG CTACCCAACT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGATAT GTTGGAGTTC GACTACACTA GACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t