Construct: ORF TRCN0000480122
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013154.1_s317c1
- Derived from:
- ccsbBroadEn_15565
- DNA Barcode:
- CATTCTGCACATTGTCCCCACCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RFX4 (5992)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480122
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5992 | RFX4 | regulatory factor X4 | NM_032491.6 | 99.8% | 99.8% | 1146_1147delGGinsAA |
| 2 | human | 5992 | RFX4 | regulatory factor X4 | NM_213594.2 | 85.3% | 83.3% | (many diffs) |
| 3 | human | 5992 | RFX4 | regulatory factor X4 | NM_001206691.2 | 84.3% | 82.3% | (many diffs) |
| 4 | mouse | 71137 | Rfx4 | regulatory factor X, 4 (inf... | NM_027689.3 | 89.4% | 95% | (many diffs) |
| 5 | mouse | 71137 | Rfx4 | regulatory factor X, 4 (inf... | XM_006514115.3 | 85.5% | 90.6% | (many diffs) |
| 6 | mouse | 71137 | Rfx4 | regulatory factor X, 4 (inf... | NM_001024918.1 | 75.9% | 80.4% | (many diffs) |
| 7 | mouse | 71137 | Rfx4 | regulatory factor X, 4 (inf... | XM_006514113.3 | 74.9% | 79.4% | (many diffs) |
| 8 | mouse | 71137 | Rfx4 | regulatory factor X, 4 (inf... | XM_006514114.3 | 74.8% | 79.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1989
- ORF length:
- 1923
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ctgggctgcc ttcggagggt ctgaattctt catcccagaa ggcattcaga 121 tagattcgag atgcccacta agcagaaata tcacggaatg gtaccattac tatggcattg 181 cagtgaaaga aagctcccaa tattatgatg tgatgtattc caagaaagga gctgcctggg 241 tgagtgagac gggcaagaaa gaagtgagca aacagacagt ggcatattca ccccggtcca 301 aactcggaac actgctgcca gaatttccca atgtcaaaga tctaaatctg ccagccagcc 361 tgcctgagga gaaggtttct acctttatta tgatgtacag aacacactgt cagagaatac 421 tggacactgt aataagagcc aactttgatg aggttcaaag tttccttctg cacttttggc 481 aaggaatgcc gccccacatg ctgcctgtgc tgggctcctc cacggtggtg aacattgtcg 541 gcgtgtgtga ctccatcctc tacaaagcta tctccggggt gctgatgccc actgtgctgc 601 aggcattacc tgacagctta actcaggtga ttcgaaagtt tgccaagcaa ctggatgagt 661 ggctaaaagt ggctctccac gacctcccag aaaacttgcg aaacatcaag ttcgaattgt 721 cgagaaggtt ctcccaaatt ctgagacggc aaacatcact aaatcatctc tgccaggcat 781 ctcgaacagt gatccacagt gcagacatca cgttccaaat gctggaagac tggaggaacg 841 tggacctgaa cagcatcacc aagcaaaccc tttacaccat ggaagactct cgcgatgagc 901 accggaaact catcacccaa ttatatcagg agtttgacca tctcttggag gagcagtctc 961 ccatcgagtc ctacattgag tggctggata ccatggttga ccgctgtgtt gtgaaggtgg 1021 ctgccaagag acaagggtcc ttgaagaaag tggcccagca gttcctcttg atgtggtcct 1081 gtttcggcac aagggtgatc cgggacatga ccttgcacag cgcccccagc ttcgggtctt 1141 ttcacctaat tcacttaatg tttgatgact acgtgctcta cctgttagaa tctctgcact 1201 gtcaggagcg aaccaatgag ctcatgcgag ccatgaaggg agaaggaagc actgcagaag 1261 tccgagaaga gatcatcttg acagaggctg ccgcaccaac cccttcacca gtgccatcgt 1321 tttctccagc aaaatctgcc acatctgtgg aagtgccacc tccctcttcc cctgttagca 1381 atccttcccc tgagtacact ggcctcagca ctacaggagc aatgcagtct tacacgtggt 1441 ctctaacata cacagtgacg acggctgctg ggtccccagc tgagaactcc caacagctgc 1501 cctgtatgag gaacactcat gtgccttctt ccTCCGTCAC ACACAGGATA CCAGTTTATC 1561 CCCACAGAGA GGAACATGGA TACACGGGAA GCTATAACTA TGGGAGCTAT GGCAACCAGC 1621 ATCCTCACCC CATGCAGAGC CAGTATCCGG CCCTCCCTCA TGACACAGCT ATCTCTGGGC 1681 CACTCCACTA TGCCCCTTAC CACAGGAGCT CTGCACAGTA CCCTTTTAAT AGCCCCACTT 1741 CCCGGATGGA ACCTTGTTTG ATGAGCAGTA CTCCCAGACT GCATCCTACC CCAGTCACTC 1801 CCCGCTGGCC AGAGGTGCCC TCAGCCAACA CGTGCTACAC AAGCCCGTCT GTGCATTCTG 1861 CGAGGTACGG AAACTCTAGT GACATGTATA CACCTCTGAC AACGCGCAGG AATTCTGAAT 1921 ATGAGCACAT GCAACACTTT CCTGGCTTTG CTTACATCAA CGGAGAGGCC TCTACAGGAT 1981 GGGCTAAATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2041 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2101 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACATTCT GCACATTGTC CCCACCCCAC 2161 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt