Construct: ORF TRCN0000480134
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006248.1_s317c1
- Derived from:
- ccsbBroadEn_03403
- DNA Barcode:
- CCCCACCACCGTACAAACCTCGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIS18A (54069)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480134
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54069 | MIS18A | MIS18 kinetochore protein A | NM_018944.3 | 100% | 100% | |
| 2 | human | 54069 | MIS18A | MIS18 kinetochore protein A | XM_017028401.1 | 84.6% | 75.9% | (many diffs) |
| 3 | human | 54069 | MIS18A | MIS18 kinetochore protein A | XM_017028400.1 | 82.9% | 73.7% | (many diffs) |
| 4 | human | 54069 | MIS18A | MIS18 kinetochore protein A | XR_002958619.1 | 13.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 765
- ORF length:
- 699
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aggcgttcgg tcactgaggt gtagcagagg atgcgctggc ggctgtgagt 121 gcggcgacaa gggcaaatgc agcgactcct cgctgttggg caagagactc tccgaagact 181 cgagccgcca ccagctgttg cagaagtggg cgagcatgtg gagctccatg agcgaagacg 241 cgtcggtggc cgacatggag agggcgcagc tggaggagga ggcggcggct gcggaggaga 301 ggccgctggt gttcctgtgc tccggctgcc ggcggccgct gggcgactcg ctgagctggg 361 tggccagcca ggaggacacc aactgcatcc TGCTTCGCTG TGTTTCCTGT AATGTTTCTG 421 TGGATAAGGA ACAGAAGCTA TCCAAACGTG AAAAGGAAAA TGGTTGCGTC CTTGAGACTT 481 TGTGCTGCGC GGGGTGCTCA CTCAATCTTG GCTACGTGTA CAGATGCACG CCCAAGAATC 541 TTGATTACAA GAGAGACTTG TTTTGCCTCA GTGTTGAAGC CATTGAAAGT TATGTTTTAG 601 GGTCCTCTGA AAAGCAAATT GTGTCAGAAG ATAAAGAGCT TTTTAATCTT GAAAGCAGAG 661 TTGAAATAGA AAAGTCTCTA ACACAGATGG AAGATGTCTT GAAAGCATTA CAAATGAAGC 721 TGTGGGAGGC CGAATCCAAA TTGTCCTTTG CCACTTGTAA AAGCTGCCCA ACTTTCTTGT 781 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 841 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 901 GAAAGGACGA CCCCACCACC GTACAAACCT CGAGACGCGT TAAGTCgaca atcaacctct 961 ggattacaaa atttgtgaaa gatt