Construct: ORF TRCN0000480139

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001932.2_s317c1
Derived from:
ccsbBroadEn_15039
DNA Barcode:
CATTGGTCGATGTTTAGTACGTCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TPK1 (27010)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480139
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350879.1 99.7% 99.5% 578_579delTGinsGT
2 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_022445.4 99.7% 99.5% 578_579delTGinsGT
3 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_005249970.1 99.7% 99.5% 578_579delTGinsGT
4 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516031.1 90% 89.9% 256_333del;656_657delTGinsGT
5 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516032.2 90% 89.9% 256_333del;656_657delTGinsGT
6 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516033.2 90% 89.9% 256_333del;656_657delTGinsGT
7 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516034.2 90% 89.9% 256_333del;656_657delTGinsGT
8 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350881.1 89.7% 86% (many diffs)
9 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516035.3 81.3% 77.6% (many diffs)
10 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011969.1 81% 73.1% (many diffs)
11 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011972.1 80% 76.5% (many diffs)
12 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001042482.1 79.5% 79.4% 354_355ins147;431_432delTGinsGT
13 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350880.1 79.5% 79.4% 354_355ins147;431_432delTGinsGT
14 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516040.2 79.3% 76.2% (many diffs)
15 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516043.1 71.8% 71.7% 256_333del;432_433ins147;509_510delTGinsGT
16 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350882.1 69.5% 76.4% 0_1ins136;48_168del;563_564delTGinsGT
17 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350883.1 69.5% 76.4% 0_1ins136;48_168del;563_564delTGinsGT
18 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350884.2 69.5% 76.4% 0_1ins136;48_168del;563_564delTGinsGT
19 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516039.2 68.3% 64% (many diffs)
20 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011974.1 68.3% 64% (many diffs)
21 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011975.1 68.3% 64% (many diffs)
22 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011980.2 66.1% 64.6% (many diffs)
23 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011981.2 66.1% 64.6% (many diffs)
24 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516037.2 63.6% 69.3% (many diffs)
25 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011970.1 63.6% 69.3% (many diffs)
26 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011971.1 63.6% 69.3% (many diffs)
27 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350885.1 56.1% 55.9% 0_1ins318;260_261delTGinsGT
28 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350886.1 56.1% 55.9% 0_1ins318;260_261delTGinsGT
29 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350887.1 56.1% 55.9% 0_1ins318;260_261delTGinsGT
30 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350889.1 56.1% 55.9% 0_1ins318;260_261delTGinsGT
31 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350893.1 56.1% 55.9% 0_1ins318;260_261delTGinsGT
32 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350894.1 56.1% 55.9% 0_1ins318;260_261delTGinsGT
33 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516047.2 56.1% 55.9% 0_1ins318;260_261delTGinsGT
34 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516048.1 56.1% 55.9% 0_1ins318;260_261delTGinsGT
35 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011982.1 56.1% 55.9% 0_1ins318;260_261delTGinsGT
36 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_024446717.1 56.1% 55.9% 0_1ins318;260_261delTGinsGT
37 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_024446715.1 52.2% 57.2% (many diffs)
38 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350895.1 51.4% 51% 0_1ins351;2T>A;227_228delTGinsGT
39 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_024446716.1 51.4% 51% 0_1ins351;2T>A;227_228delTGinsGT
40 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516046.1 44.4% 43.8% (many diffs)
41 human 27010 TPK1 thiamin pyrophosphokinase 1 XR_001744630.1 35.5% (many diffs)
42 human 27010 TPK1 thiamin pyrophosphokinase 1 NR_146936.2 24.1% (many diffs)
43 human 27010 TPK1 thiamin pyrophosphokinase 1 NR_146934.1 23.6% (many diffs)
44 mouse 29807 Tpk1 thiamine pyrophosphokinase NM_013861.4 86.8% 89.3% (many diffs)
45 mouse 29807 Tpk1 thiamine pyrophosphokinase XM_006506219.3 78.9% 81% (many diffs)
46 mouse 29807 Tpk1 thiamine pyrophosphokinase NM_001311111.1 70% 70.7% (many diffs)
47 mouse 29807 Tpk1 thiamine pyrophosphokinase XM_006506218.3 67.2% 66.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
795
ORF length:
729
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gcatgccttt accccgttgg agcccctgct ttccactggg aatttgaagt
121actgccttgt aattcttaat cagcctttgg acaactattt tcgtcatctt tggaacaaag
181ctcttttaag agcctgtgcc gatggaggtg ccaaccgctt atatgatatc accgaaggag
241agagagaaag ctttttgcct gaattcatca atggagactt tgattctatt aggcctgaag
301tcagagaata ctatgctact aagggatgtg agctcatttc aactcctgat caagaccaca
361ctgactttac taagtgccTT AAAATGCTCC AAAAGAAGAT AGAAGAAAAA GACTTAAAGG
421TTGATGTGAT CGTGACACTG GGAGGCCTTG CTGGGCGTTT TGACCAGATT ATGGCATCTG
481TGAATACCTT GTTCCAAGCG ACTCACATCA CTCCTTTTCC AATTATAATA ATCCAAGAGG
541AATCGCTGAT CTACCTGCTC CAACCAGGAA AGCACAGGTT GCATGTAGAC ACTGGAATGG
601AGGGTGATTG GTGTGGCCTT ATTCCTGTTG GACAGCCTTG TAGTCAGGTT ACAACCACAG
661GCCTCAAGTG GAACCTCACA AATGATGTGC TTGCTTTTGG AACATTGGTC AGTACTTCCA
721ATACCTACGA CGGGTCTGGT GTTGTGACTG TGGAAACTGA CCACCCACTC CTCTGGACCA
781TGGCCATCAA AAGCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
841CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
901TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CATTGGTCGA TGTTTAGTAC
961GTCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)